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Sample GSM126441 Query DataSets for GSM126441
Status Public on Jul 31, 2008
Title mammary gland-2ug 17-beta-estrogen + 1mg progesterone intraperitoneally-biologic replicate 1 MG-U74B
Sample type RNA
 
Source name mammary gland
Organism Mus musculus
Characteristics eight week old virgin BALB/cJ mice were ovariectomized followed by a period of one week before treatment to clear endogenous hormones
lymph node-free mammary gland
Treatment protocol ovariectomized animals were injected daily with 2ug 17-Beta-estrogen + 1 mg progesterone for 4 days
Extracted molecule total RNA
Label biotin
Label protocol Biotinylated cRNA were prepared according to standard Affymetrix protocol from 2ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Following fragmentation, 10ug of cRNA were hybridized for 16 hr at 45C on Affymetrix U74A GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500.
Description Mammary gland mRNA expression data from mice treated with progesterone.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 150.
 
Submission date Aug 09, 2006
Last update date Dec 27, 2007
Contact name D. Joseph Jerry
E-mail(s) jjerry@vasci.umass.edu
Phone 413-545-5335
Fax 413-545-6326
URL http://htpp://www.umass.edu/vasci/faculty/jerry/jerry.html
Organization name University of Massachusetts
Department Veterinary & Animal Scineces
Street address 161 Holdsworth Way
City Amherst
State/province MA
ZIP/Postal code 01003
Country USA
 
Platform ID GPL82
Series (1)
GSE5483 Transcriptional Profiling of Responses to Estrogen and Progesterone in Mammary Gland

Data table header descriptions
ID_REF Affymetrix probeset identifier
VALUE MAS5 calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or Marginal (M).
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
104769_at 29 A 0.107301
104770_at 25.4 A 0.621835
104771_at 10.7 A 0.94553
104772_at 129.7 M 0.05447
104773_at 4.8 A 0.581931
104774_at 49.4 A 0.189687
104775_at 255.7 P 0.014937
104776_at 47.5 A 0.302547
104777_at 4 A 0.581944
104778_at 61.4 M 0.043968
104779_at 85.5 A 0.541184
104780_at 66.3 A 0.302547
104781_at 5.9 A 0.660442
104782_at 343.2 P 0.002228
104783_at 71.3 A 0.284747
104784_at 55.3 A 0.250724
104785_at 176.4 A 0.07383
104786_at 58.3 A 0.218983
104787_at 14.1 A 0.732537
104788_at 53.2 A 0.378184

Total number of rows: 12477

Table truncated, full table size 320 Kbytes.




Supplementary data files not provided

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