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Sample GSM126459 Query DataSets for GSM126459
Status Public on Jul 31, 2008
Title mammary gland-1mg progesterone intraperitoneally-biologic replicate 3 MG-U74C
Sample type RNA
 
Source name mammary gland
Organism Mus musculus
Characteristics eight week old virgin BALB/cJ mice were ovariectomized followed by a period of one week before treatment to clear endogenous hormones
lymph node-free mammary gland
Treatment protocol ovariectomized animals were injected daily with 1 mg progesterone for 4 days
Extracted molecule total RNA
Extraction protocol Ultraspec for RNA extraction (Biotexc, Houston, TX)
Label biotin
Label protocol Biotinylated cRNA were prepared according to standard Affymetrix protocol from 2ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Following fragmentation, 10ug of cRNA were hybridized for 16 hr at 45C on Affymetrix U74A GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500.
Description Mammary gland mRNA expression data from mice treated with progesterone.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 150.
 
Submission date Aug 09, 2006
Last update date Dec 27, 2007
Contact name D. Joseph Jerry
E-mail(s) jjerry@vasci.umass.edu
Phone 413-545-5335
Fax 413-545-6326
URL http://htpp://www.umass.edu/vasci/faculty/jerry/jerry.html
Organization name University of Massachusetts
Department Veterinary & Animal Scineces
Street address 161 Holdsworth Way
City Amherst
State/province MA
ZIP/Postal code 01003
Country USA
 
Platform ID GPL83
Series (1)
GSE5483 Transcriptional Profiling of Responses to Estrogen and Progesterone in Mammary Gland

Data table header descriptions
ID_REF Affymetrix probeset identifier
VALUE MAS5 calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or Marginal (M).
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
128520_at 1573.6 A 0.107301
128523_at 9.7 A 0.849473
128529_at 254.3 A 0.32083
128550_at 76.2 A 0.541184
128554_at 118.3 A 0.621816
128577_s_at 101.2 A 0.204022
128578_r_at 389.5 P 0.005643
128579_r_at 123.6 A 0.234557
128580_at 22.8 A 0.749276
128582_at 66.8 A 0.378184
128583_at 73.1 P 0.003067
128590_at 212.6 A 0.204022
128624_at 10.1 A 0.910595
128643_r_at 431.3 A 0.302547
128644_at 444.8 A 0.060419
128645_at 119 A 0.438361
128646_at 206.3 P 0.024711
128647_at 148.1 A 0.218983
128651_at 266.4 A 0.284747
128652_at 45.9 A 0.541184

Total number of rows: 11934

Table truncated, full table size 310 Kbytes.




Supplementary data files not provided

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