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Sample GSM126461 Query DataSets for GSM126461
Status Public on Jul 31, 2008
Title mammary gland-2ug 17-beta-estrogen + 1mg progesterone intraperitoneally-biologic replicate 1 MG-U74C
Sample type RNA
 
Source name mammary gland
Organism Mus musculus
Characteristics eight week old virgin BALB/cJ mice were ovariectomized followed by a period of one week before treatment to clear endogenous hormones
lymph node-free mammary gland
Treatment protocol ovariectomized animals were injected daily with 2ug 17-Beta-estrogen + 1 mg progesterone for 4 days
Extracted molecule total RNA
Label biotin
Label protocol Biotinylated cRNA were prepared according to standard Affymetrix protocol from 2ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
 
Hybridization protocol Following fragmentation, 10ug of cRNA were hybridized for 16 hr at 45C on Affymetrix U74A GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500.
Description Mammary gland mRNA expression data from mice treated with progesterone.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was set to 150.
 
Submission date Aug 09, 2006
Last update date Dec 27, 2007
Contact name D. Joseph Jerry
E-mail(s) jjerry@vasci.umass.edu
Phone 413-545-5335
Fax 413-545-6326
URL http://htpp://www.umass.edu/vasci/faculty/jerry/jerry.html
Organization name University of Massachusetts
Department Veterinary & Animal Scineces
Street address 161 Holdsworth Way
City Amherst
State/province MA
ZIP/Postal code 01003
Country USA
 
Platform ID GPL83
Series (1)
GSE5483 Transcriptional Profiling of Responses to Estrogen and Progesterone in Mammary Gland

Data table header descriptions
ID_REF Affymetrix probeset identifier
VALUE MAS5 calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or Marginal (M).
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
128520_at 1201.8 A 0.162935
128523_at 70.6 M 0.048995
128529_at 158 A 0.52062
128550_at 28.3 A 0.795978
128554_at 115 A 0.397994
128577_s_at 94.4 A 0.250724
128578_r_at 308 P 0.021866
128579_r_at 13.4 A 0.824011
128580_at 20.7 A 0.781017
128582_at 59.2 A 0.5
128583_at 142.4 P 0.004863
128590_at 265 A 0.284747
128624_at 13.1 A 0.795978
128643_r_at 429.5 A 0.162935
128644_at 316.5 A 0.089405
128645_at 133 A 0.541184
128646_at 109.6 A 0.267463
128647_at 562.6 P 0.019304
128651_at 284.9 A 0.302547
128652_at 45.7 A 0.697453

Total number of rows: 11934

Table truncated, full table size 310 Kbytes.




Supplementary data files not provided

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