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Sample GSM1265694 Query DataSets for GSM1265694
Status Public on Dec 31, 2014
Title muscle cell line (C2C12) at day 0 replication 1
Sample type RNA
 
Source name C2C12
Organism Mus musculus
Characteristics cell line: C2C12
time: day 0
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Qiagen miReasy Mini kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol for small-RNA. Then small RNA was cleaned up using the Rneasy MinElute Clean up kit (Qiagen, Hilden, Germany).
Label biotin
Label protocol Biotinylated cRNA were prepared from miRNA according to FlashTag™ Biotin RNA Labeling Kit for Affymetrix GeneChip miRNA arrays (Genisphere, Hatfield, PA, USA) according to the manufacturer’s recommendations Affymetrix® GeneChip® miRNA Array protocols (Affymetrix, Santa Clara, CA, USA)
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42C on Affymetrix GeneChip miRNA arrays. GeneChips were washed and processed to detect biotin-containing transcripts by Streptavidin-PE (Phycoerythrin) conjugate.
Scan protocol Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
Description replication 1
miRNA expression data from mouse muscle lcell line
Data processing Data were analysed with Expression Console and quantify by med polish
 
Submission date Nov 15, 2013
Last update date Dec 31, 2014
Contact name Klaus Wimmers
E-mail(s) s.wimmers@fbn-dummerstorf.de
Phone 0049 3820868700
Organization name Leibniz Institute for Farm Animal Biology (FBN)
Street address Wilhelm-Stahl-Allee 2
City Dummerstorf
ZIP/Postal code 18196
Country Germany
 
Platform ID GPL16384
Series (1)
GSE52410 Gene expression data of micro RNA from mouse muscle cell line C2C12

Data table header descriptions
ID_REF
VALUE RMA algorithm in log2 scale

Data table
ID_REF VALUE
14q0_st 0.54
14qI-1_st 2.02
14qI-1_x_st 1.42
14qI-2_st 0.61
14qI-3_x_st 0.57
14qI-4_st 0.5
14qI-4_x_st 0.47
14qI-5_st 0.62
14qI-6_st 0.69
14qI-6_x_st 0.66
14qI-7_st 1.02
14qI-8_st 0.78
14qI-8_x_st 1.1
14qI-9_x_st 0.71
14qII-10_st 0.74
14qII-10_x_st 0.51
14qII-11_st 0.62
14qII-11_x_st 0.5
14qII-12_st 1.01
14qII-12_x_st 0.98

Total number of rows: 25015

Table truncated, full table size 536 Kbytes.




Supplementary file Size Download File type/resource
GSM1265694_C2C12P8R1_D0.CEL.gz 817.3 Kb (ftp)(http) CEL
Processed data included within Sample table

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