|
| Status |
Public on Aug 01, 2014 |
| Title |
Small Intestine goblet cell 1 |
| Sample type |
RNA |
| |
|
| Source name |
Small Intestine goblet cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
tissue: small intesine
cell type: isolated epithelial cells
cell subtype: FACS sorted goblet cells (CD45-CD24-CK18+UEA-I+ cells)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were sorted directly into Qiagen buffer RLT. RNA was extracted using a Qiagen RNAeasy mini kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 1
|
| Data processing |
The data were normalised using average normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Nov 15, 2013 |
| Last update date |
Aug 01, 2014 |
| Contact name |
Kathryn A Knoop |
| E-mail(s) |
knoop.kathryn@mayo.edu
|
| Organization name |
Mayo Clinic
|
| Department |
Internal Medicine, Gastroenterology Division
|
| Street address |
2511 Boulder Ridge Dr NW
|
| City |
ROCHESTER |
| State/province |
MN |
| ZIP/Postal code |
55901 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE52418 |
Gene expression in small intestinal and colonic goblet cells from C57Bl/6 mice |
|
