|
| Status |
Public on Dec 18, 2013 |
| Title |
Ad-GFP infected liver, PTU fed and saline treated, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
liver, Ad-GFP, saline
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: FVB
genotype/variation: BirA+/-
tissue: liver
infection: Ad-GFP virus
treatment: saline
|
| Treatment protocol |
Mice were fed PTU diet for 3 weeks, followed by adenoviral injections through the tail vein. Mice were then injected intraperitoneally with saline or T3 for 4 days and then euthanized and tissues were collected.
|
| Growth protocol |
9- to 10-week-old heterozygous BirA-expressing mice were housed in individual cages, fed a PTU diet, administered either Ad-GFP or Ad-TRb virus and then treated with either saline or T3.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Perfect Pure RNA Tissue kit from 5 Prime.
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA were amplified and labeled using the Ambion Whole Transcript Expression Kit (Catalog Number 4425209, Ambion, Austin, TX) and Affymetix GeneChip Whole Transcript Terminal Labeling Kit (Catalog Number 900671, Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip® Mouse Gene 2.0 ST arrays were washed, stained and scanned according to the protocol described in GeneChip® WT Terminal Labeling and Hybridization User Manual (Fluidics Protocol FS450_0002).
|
| Scan protocol |
Scanning was done on an Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression from mouse liver infected with Ad-GFP virus, PTU-fed and saline treated.
Jackson Labs' male FVB;129P2-Gt(ROSA)26Sortm1(birA)Mejr/J homozygotes were crossed to pure FVB female mice, and the heterozygote offspring were used for experiments/subsequent breeding.
|
| Data processing |
RMA (Robust Multiarray average) Signal extraction using Affymetrix Expression Console Version 1.2.1.20.
|
| |
|
| Submission date |
Nov 15, 2013 |
| Last update date |
Dec 18, 2013 |
| Contact name |
Preeti Ramados |
| Organization name |
Beth Israel Deaconess Medical Center
|
| Street address |
3 Blackfan Cir
|
| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE52433 |
Expression data from mouse liver over-expressing either GFP alone or biotinylated-TRb1 and GFP |
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