|
| Status |
Public on Jan 19, 2014 |
| Title |
skin_SRSF6, biological rep1 (exon-level) |
| Sample type |
RNA |
| |
|
| Source name |
SRSF6 expressing skin, d21
|
| Organism |
Mus musculus |
| Characteristics |
tissue: skin
genotype/variation: tg (R26-rtTA+/-, ColA1-TREtight-SRSF6+/-)
doxycycline: dox
|
| Treatment protocol |
1) doxycycline-treated/SRSF6 expressing animals (for 21 days) or 2) controls
|
| Growth protocol |
Mouse skin from trangenic animals, age 6 weeks or more
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Skin tissues were homogenized using liquid nitrogen, and total RNA was isolated using an RNeasy Mini Kit (Qiagen). RNA quality was determined on an Agilent 2100 Bioanalyzer; samples with a RIN score of ≥7.0 passed the quality control.
|
| Label |
3+
|
| Label protocol |
RNA was amplified, and converted into cDNA using a WT Expression kit (Ambion). This was followed by cDNA fragmentation and terminal biotin-labeling, using a GeneChip WT Terminal Labeling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were then hybridized, washed, and scanned according to the manufacturer’s instructions, using Mouse Gene ST 1.0 GeneChips (Affymetrix).
|
| Scan protocol |
Affymetrix Gene ChIP Scanner
|
| Description |
AKMJ052410_Y1+SK_D21.CEL
|
| Data processing |
Raw data were normalized, and analyzed for gene expression and AS in parallel, using AltAnalyze version 1.0 software. The splicing index method was used to calculate AS for pairwise comparisons. Putative AS events were identified as common identities from two independent pairwise comparisons.
library-file: MoGene-1_0-st-v1.r4.pgf
annotation-file: MoGene-1_0-st-v1.na30.1.mm9.probeset.csv
|
| |
|
| Submission date |
Nov 18, 2013 |
| Last update date |
Jan 19, 2014 |
| Contact name |
Mads A Jensen |
| Organization name |
Cold Spring Harbor Laboratory
|
| Street address |
1 Bungtown Road
|
| City |
Cold Spring Harbor |
| State/province |
New York |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platform ID |
GPL10740 |
| Series (1) |
| GSE52458 |
Overexpression of splicing factor SRSF6 in mouse skin |
|
| Relations |
| Alternative to |
GSM1274322 (gene-level analysis) |
