|
| Status |
Public on Dec 05, 2013 |
| Title |
BS2011051056 |
| Sample type |
RNA |
| |
|
| Source name |
Gastrocnemius muscle mRNA from young (10 week old) knock-out mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Gastrocnemius muscle
genotype: KO
age: YOUNG
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
standard Affymetrix procedure.
|
| Scan protocol |
standard Affymetrix procedure.
|
| Description |
Gene expression data of gastrocnemius muscle mRNA from young (10 week old) knock-out mice
|
| Data processing |
The data were analyzed with RMA (log2) normalized data using the just.rma function in the affy package in Biocinductor
|
| |
|
| Submission date |
Nov 20, 2013 |
| Last update date |
Dec 05, 2013 |
| Contact name |
Benjamin Haibe-Kains |
| E-mail(s) |
benjamin.haibe.kains@utoronto.ca
|
| Phone |
+14165818626
|
| Organization name |
Princess Margaret Cancer Centre
|
| Department |
Princess Margaret Research
|
| Lab |
Bioinformatics and Computational Genomics
|
| Street address |
610 University Avenue
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 2M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE52550 |
Progressive loss of PGC-1alpha expression in aging muscle potentiates glucose intolerance and systemic inflammation |
|
