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Sample GSM127022 Query DataSets for GSM127022
Status Public on Sep 01, 2006
Title LNCaP cells, treated with 20uM gedunin for 6h, biol repl 3
Sample type RNA
 
Source name LNCaP cell line
Organism Homo sapiens
Characteristics = LNCaP cells, treated with 20uM gedunin for 6h
Treatment protocol LNCaP cells grown in RPMI and 10%FBS were treated with 20uM gedunin for 6h
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Following fragmentation, cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained on the Affymetrix High-throughput array platform
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner
Description Gene expression data from LNCaP cells treated with 20uM gedunin for 6h
Data processing The data were processed by RMA (GenePattern)
 
Submission date Aug 11, 2006
Last update date Aug 11, 2006
Contact name Haley Hieronymus
E-mail(s) haley@broad.mit.edu
URL http://www.broad.mit.edu/cgi-bin/cancer/publications/pub_menu.cgi
Organization name Broad Institute of Harvard and MIT
Street address 7 Cambridge Center
City CAMBRIDGE
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL3921
Series (1)
GSE5506 LNCaP cells treated with gedunin or vehicle for 6h

Data table header descriptions
ID_REF ID
VALUE RMA-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
1007_s_at 1273.312419 P
1053_at 78.7202355 P
117_at 943.740223 P
121_at 58.70873261 P
1255_g_at 14.28268867 A
1294_at 20.78013636 A
1316_at 17.75698937 A
1320_at 17.07823721 A
1405_i_at 17.11447209 A
1431_at 20.24425599 A
1438_at 36.93440074 P
1487_at 52.15432192 P
1494_f_at 23.49939399 A
1598_g_at 52.80501289 P
160020_at 24.56975154 A
1729_at 73.68736836 P
1773_at 25.72048821 A
177_at 17.51485651 A
179_at 57.73871447 A
1861_at 120.3214537 P

Total number of rows: 22215

Table truncated, full table size 538 Kbytes.




Supplementary data files not provided

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