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Sample GSM127034 Query DataSets for GSM127034
Status Public on Sep 01, 2006
Title Androgen-starved LNCaP cells, treated with 1nM R1881 and DMSO for 6h, biol repl 2 (celastrol)
Sample type RNA
 
Source name LNCaP cell line
Organism Homo sapiens
Characteristics androgen-starved LNCaP cells, treated with 1nM R1881 and DMSO for 6h
Treatment protocol LNCaP cells were grown in RPMI and charcol-stripped 10%FBS for 2d and then treated with 1nM R1881 and DMSO for 24h
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Following fragmentation, cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained on a Affymetrix High-throughput array platform
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner
Description Gene expression data from androgen-starved LNCaP cells treated with 1nM R1881 and DMSO for 24h
Data processing The data were processed by RMA (GenePattern)
 
Submission date Aug 11, 2006
Last update date Aug 11, 2006
Contact name Haley Hieronymus
E-mail(s) haley@broad.mit.edu
URL http://www.broad.mit.edu/cgi-bin/cancer/publications/pub_menu.cgi
Organization name Broad Institute of Harvard and MIT
Street address 7 Cambridge Center
City CAMBRIDGE
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL3921
Series (1)
GSE5507 Androgen-starved LNCaP cells treated with celastrol and androgen, androgen alone, or vehicle alone for 24h

Data table header descriptions
ID_REF ID
VALUE RMA-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
1007_s_at 1414.65 P
1053_at 335.217 P
117_at 30.528 A
121_at 113.329 P
1255_g_at 24.329 A
1294_at 37.205 A
1316_at 38.129 A
1320_at 72.213 P
1405_i_at 41.463 A
1431_at 57.741 P
1438_at 104.958 P
1487_at 168.024 P
1494_f_at 44.183 A
1598_g_at 104.003 P
160020_at 57.904 A
1729_at 417.887 P
1773_at 68.899 P
177_at 25.881 A
179_at 144.194 A
1861_at 283.766 P

Total number of rows: 22215

Table truncated, full table size 442 Kbytes.




Supplementary data files not provided

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