|
| Status |
Public on Sep 01, 2006 |
| Title |
LNCaP cells treated with 1nM R1881 and 20uM gedunin for 24h, biol repl 2 |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP cell line
|
| Organism |
Homo sapiens |
| Characteristics |
LNCaP cells treated with 1nM R1881 and 20uM gedunin for 24h
|
| Treatment protocol |
LNCaP cells were grown in RPMI and charcol-stripped 10%FBS for 2d and then treated with 20uM gedunin plus 1nM R1881 (synthetic, non-metabolizable androgen) for 24h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained on a Affymetrix Fluidics station
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner
|
| Description |
Gene expression data from androgen-starved LNCaP cells treated with 1with 1nM R1881 and 20uM gedunin for 24h
|
| Data processing |
The data were processed by RMA (GenePattern)
|
| |
|
| Submission date |
Aug 11, 2006 |
| Last update date |
Aug 11, 2006 |
| Contact name |
Haley Hieronymus |
| E-mail(s) |
haley@broad.mit.edu
|
| URL |
http://www.broad.mit.edu/cgi-bin/cancer/publications/pub_menu.cgi
|
| Organization name |
Broad Institute of Harvard and MIT
|
| Street address |
7 Cambridge Center
|
| City |
CAMBRIDGE |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE5508 |
Androgen-starved LNCaP cells treated with gedunin and androgen, androgen alone, or vehicle alone for 24h |
|
