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Sample GSM1273850 Query DataSets for GSM1273850
Status Public on Apr 24, 2015
Title Foxo 1,3,4 muscle specific knock-out, fed
Sample type RNA
 
Source name gastrocnemius muscle of fed ko mice
Organism Mus musculus
Characteristics genotype/variation: knocked-out FoxO 1,3,4
strain: cross of C57BL/6N and FVBN
gender: male
Treatment protocol animals were starved 24 hours with water ad libitum, control animals had both food and water ad libitum
Growth protocol animals were kept on standard 12h dark, 12 h light cycles, food an water was given ad libitum except for the starvation experiments
Extracted molecule total RNA
Extraction protocol Muscles were frozen in liquid nitrogen, disrupted using mortar and pescle and RNA was extracted using the standard Trizol method. RNA quality was monitored by UV absorption and Agilent Bioanalyzer.
Label biotin
Label protocol Standard Afymetrix Procedure
 
Hybridization protocol Standard Afymetrix Procedure
Scan protocol Standard Afymetrix Procedure
Data processing Data was summarized using the MAS 5.0 algorithm in Affymetrix Expression Console.
 
Submission date Nov 22, 2013
Last update date Apr 24, 2015
Contact name Marco Sandri
E-mail(s) marco.sandri@unipd.it
Organization name University of Padova
Department Department of Biomedical Science
Street address Viale Colombo 3
City Padova
ZIP/Postal code 35121
Country Italy
 
Platform ID GPL1261
Series (1)
GSE52667 The FoxO signature in protein breakdown

Data table header descriptions
ID_REF
VALUE MAS 5.0 signal intensity and expression call
Detection

Data table
ID_REF VALUE Detection
1420147_at 2 A
1415670_at 258.7 P
1415671_at 616.8 P
1415672_at 1263.5 P
1415673_at 269.9 P
1415674_a_at 481.7 P
1415675_at 133.1 P
1415676_a_at 1959.5 P
1415677_at 143.7 P
1415678_at 1643.4 P
1415679_at 1880 P
1415680_at 155.6 P
1415681_at 897.4 P
1415682_at 215.4 P
1415683_at 1095.9 P
1415684_at 197.9 P
1415685_at 608.5 P
1415686_at 565.4 P
1415687_a_at 2315.9 P
1415688_at 2491.8 P

Total number of rows: 45101

Table truncated, full table size 802 Kbytes.




Supplementary file Size Download File type/resource
GSM1273850_EXP_FED_KO.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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