|
| Status |
Public on Apr 24, 2015 |
| Title |
Foxo 1,3,4 muscle specific knock-out, fed |
| Sample type |
RNA |
| |
|
| Source name |
gastrocnemius muscle of fed ko mice
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: knocked-out FoxO 1,3,4
strain: cross of C57BL/6N and FVBN
gender: male
|
| Treatment protocol |
animals were starved 24 hours with water ad libitum, control animals had both food and water ad libitum
|
| Growth protocol |
animals were kept on standard 12h dark, 12 h light cycles, food an water was given ad libitum except for the starvation experiments
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Muscles were frozen in liquid nitrogen, disrupted using mortar and pescle and RNA was extracted using the standard Trizol method. RNA quality was monitored by UV absorption and Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Standard Afymetrix Procedure
|
| |
|
| Hybridization protocol |
Standard Afymetrix Procedure
|
| Scan protocol |
Standard Afymetrix Procedure
|
| Data processing |
Data was summarized using the MAS 5.0 algorithm in Affymetrix Expression Console.
|
| |
|
| Submission date |
Nov 22, 2013 |
| Last update date |
Apr 24, 2015 |
| Contact name |
Marco Sandri |
| E-mail(s) |
marco.sandri@unipd.it
|
| Organization name |
University of Padova
|
| Department |
Department of Biomedical Science
|
| Street address |
Viale Colombo 3
|
| City |
Padova |
| ZIP/Postal code |
35121 |
| Country |
Italy |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE52667 |
The FoxO signature in protein breakdown |
|
