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Sample GSM1273851 Query DataSets for GSM1273851
Status Public on Apr 24, 2015
Title Foxo 1,3,4 muscle specific knock-out, starved
Sample type RNA
 
Source name gastrocnemius muscle of starved ko mice
Organism Mus musculus
Characteristics genotype/variation: knocked-out FoxO 1,3,4
strain: cross of C57BL/6N and FVBN
gender: male
Treatment protocol animals were starved 24 hours with water ad libitum, control animals had both food and water ad libitum
Growth protocol animals were kept on standard 12h dark, 12 h light cycles, food an water was given ad libitum except for the starvation experiments
Extracted molecule total RNA
Extraction protocol Muscles were frozen in liquid nitrogen, disrupted using mortar and pescle and RNA was extracted using the standard Trizol method. RNA quality was monitored by UV absorption and Agilent Bioanalyzer.
Label biotin
Label protocol Standard Afymetrix Procedure
 
Hybridization protocol Standard Afymetrix Procedure
Scan protocol Standard Afymetrix Procedure
Data processing Data was summarized using the MAS 5.0 algorithm in Affymetrix Expression Console.
 
Submission date Nov 22, 2013
Last update date Apr 24, 2015
Contact name Marco Sandri
E-mail(s) marco.sandri@unipd.it
Organization name University of Padova
Department Department of Biomedical Science
Street address Viale Colombo 3
City Padova
ZIP/Postal code 35121
Country Italy
 
Platform ID GPL1261
Series (1)
GSE52667 The FoxO signature in protein breakdown

Data table header descriptions
ID_REF
VALUE MAS 5.0 signal intensity and expression call
Detection

Data table
ID_REF VALUE Detection
1420147_at 1 A
1415670_at 233.1 P
1415671_at 596.6 P
1415672_at 1142.1 P
1415673_at 198.3 P
1415674_a_at 514.4 P
1415675_at 133.9 P
1415676_a_at 1761.2 P
1415677_at 141.8 P
1415678_at 1525.7 P
1415679_at 2195.1 P
1415680_at 186.3 P
1415681_at 888.2 P
1415682_at 195.3 P
1415683_at 1270 P
1415684_at 190.8 P
1415685_at 509.7 P
1415686_at 642.1 P
1415687_a_at 2630.7 P
1415688_at 2822 P

Total number of rows: 45101

Table truncated, full table size 802 Kbytes.




Supplementary file Size Download File type/resource
GSM1273851_EXP_STARVED_KO.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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