|
| Status |
Public on Feb 05, 2014 |
| Title |
H1299_Ad-LacZ |
| Sample type |
RNA |
| |
|
| Source name |
H1299_Ad-LacZ
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H1299
cell type: human lung carcinoma cells
infected with: adenoviruses expressing LacZ
|
| Treatment protocol |
H1299 cells were infected with adenoviruses expressing LacZ, p53, p63a, p63g, p73a or p73b at 25 moi.
|
| Growth protocol |
H1299 were cultured in RPMI1640 medium with 10% FCS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
24h after infection, total RNA was extracted by RNeasy mini kit (QIAGEN) according to manufacturer's protocol. RNA was quantified using the NanoDrop spectrophotometer.
|
| Label |
Cy3
|
| Label protocol |
Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
|
| |
|
| Hybridization protocol |
Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
| Scan protocol |
Array was scanned with an Agilent G2565BA Microarray Scanner.
|
| Description |
Gene expression after Ad-LacZ infection
H1299_1
|
| Data processing |
Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
The values in the data matrix (log2) were calculated by Limma (R package) for the normalization using all probes and then the values for lincRNAs were selected for further analysis.
|
| |
|
| Submission date |
Nov 26, 2013 |
| Last update date |
Feb 05, 2014 |
| Contact name |
Masashi Idogawa |
| Organization name |
Sapporo Medical University
|
| Street address |
S1W17
|
| City |
Sapporo |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE52739 |
The effect on lincRNA expression by p53 family members. |
|
