|
| Status |
Public on Oct 13, 2014 |
| Title |
Mdx skeletal muscle, PBS treatment for 7 weeks rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Mdx skeletal muscle_PBS treatment for 7 wks
|
| Organism |
Mus musculus |
| Characteristics |
strain: Mdx
gender: male
age: 6-7 weeks
sample group: PBS treatment for 7 weeks
tissue: gastrocnemius muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Gastrocnemius muscle was pulverized and homogenized in liquid nitrogen and exactly 70 mg of muscle powder was weighted into Trizol reagent (Invitrogen, Carlsbad, CA, USA). Total RNA and DNA fractions were extracted according to the manufacturer’s guidelines. The RNA and DNA concentrations were analyzed with Nanodrop ND-1000 (Thermo Fisher Scientific Inc., Waltham MA, USA) in duplicate. The quality of RNA was confirmed by spectrophotometry (OD260/OD280 ratio of 1.9 to 2.0) and agarose gel electrophoresis. Possible DNA still left was further degraded using DNAse according to the manufacturer’s instructions (TURBO DNA-free™ Kit, Applied Biosystems by Life Technologies, USA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with Illumina TotalPrep RNA Amplification Kit (Applied Biosystems cat # AMIL1791).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 16
|
| Data processing |
The data were normalized with quantiles normalization (including log2-transformation of the data) and data quality was assessed using Chipster software (IT Center for Science, Espoo, Finland)
|
| |
|
| Submission date |
Nov 26, 2013 |
| Last update date |
Oct 13, 2014 |
| Contact name |
Juha J Hulmi |
| E-mail(s) |
juha.hulmi@jyu.fi
|
| Organization name |
University of Jyväskylä
|
| Department |
Biology of Physical Activity
|
| Street address |
Rautpohjankatu 8a Viv
|
| City |
Jyväskylä |
| ZIP/Postal code |
40014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE52766 |
The effects of muscular dystrophy, exercise and sActRIIB-Fc on molecular signature of skeletal muscle in mice |
|
