|
| Status |
Public on Dec 04, 2013 |
| Title |
T3M-1_wildtype_GFPsorted_triplicate |
| Sample type |
RNA |
| |
|
| Source name |
Cell line, wildtype infected, GFP-sorted
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: T3M-1 Cl-10
histology: squamous cell carcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the RNeasy mini kit (Qiagen) following the manufacturer's recommendations.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 0.1 ug RNA using the one-color Low Input Quick Amp Labeling Kit (Agilent) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Cy3-labelled cRNA hybridized to SurePrint G3 Human GE v2 8x60K Microarray with Gene Expression Hybridization Kit (Agilent) and Gene Expression Wash Pack (Agilent) according to the manufacturer's instructions.
|
| Scan protocol |
Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting.
|
| Description |
Gene expression 2days after retroviral infection
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.5.3 (Agilent) using default parameters. The data were log2-transformed, and normalized by percentile shift-75%. In the matrix file, mean values were calculated for each biological triplicate.
|
| |
|
| Submission date |
Dec 02, 2013 |
| Last update date |
Dec 04, 2013 |
| Contact name |
Mizuo Ando |
| E-mail(s) |
ando.hnc@gmail.com
|
| Phone |
03-3815-5411
|
| Organization name |
The University of Tokyo
|
| Department |
Otolaryngology-Head and Neck Surgery
|
| Street address |
7-3-1 Hongo
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE52855 |
Gene expression signatures for ELF4 |
|
