|
| Status |
Public on Sep 01, 2017 |
| Title |
Lung_epithelial_cells_PLY_rep.3 |
| Sample type |
RNA |
| |
|
| Source name |
AT II cells, PLY
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: female
age: 8 to 12 weeks
cell type: alveolar type II cells
treatment: PLY
|
| Treatment protocol |
Replication-deficient adenoviral vectors carrying the gene for active TGF-b1 (AdTGF-b1) were prepared. Mice received AdTGFb-1 for 14 days, and then received daily intratracheal instillations for three consecutive days of either cytotoxic PLY, or its non-cytotoxic derivative, PdB to investigate its effect on lung fibrosis perpetuation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from purified type II alveolar epithelial cells of AdTGF-b1+PLY versus AdTGF-b1+PdB treated mice using the RNeasy minikit (Qiagen, Germany).
|
| Label |
biotin
|
| Label protocol |
Biotinylated probes were prepared from 500 ng of total RNA, using the TargetAmp™-Nano Labeling Kit for Illumina® Expression BeadChip®.
|
| |
|
| Hybridization protocol |
Standard hybridization protocol for BeadArray Reader 500 X from Illumina® (San Diego, CA, USA)
|
| Scan protocol |
Standard Illumina scan protocol
|
| Description |
Mm AT II cells, PLY
|
| Data processing |
Robust spline normalization (rsn) and detection filtering via lumi package within Bioconductor and R
|
| |
|
| Submission date |
Dec 02, 2013 |
| Last update date |
Sep 01, 2017 |
| Contact name |
S. Lambeck |
| Organization name |
JUH
|
| Street address |
Erlanger Allee 101
|
| City |
Jena |
| ZIP/Postal code |
07743 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE52890 |
Infection with Streptococcus pneumoniae triggers progression of pulmonary fibrosis by pneumolysin |
|
