|
| Status |
Public on Jun 04, 2015 |
| Title |
R154(CytoScanHD_Array) |
| Sample type |
genomic |
| |
|
| Source name |
Cervical cancer biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
sample group: Case
age (years): 42
histology: SCC
tumor stage: IIIB
|
| Treatment protocol |
None
|
| Growth protocol |
None
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA extraction was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated DNA were prepared according to the standard Affymetrix protocol from 500 ng total DNA (Cytoscan HD Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, ssDNA were hybridized for 18 hr at 50C and 60 rpm on Cytoscan HD Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 protocol
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix) with GeneChip Scanner 3000
|
| Description |
Copy Number data from Cervical cancer biopsy
|
| Data processing |
Microarrays were normalized with the Chromosome Analysis Suite (ChAS) Software version 2.0 and compared with the Reference data set included in ChAS.
|
| |
|
| Submission date |
Dec 02, 2013 |
| Last update date |
Jun 04, 2015 |
| Contact name |
INGRID MEDINA MARTINEZ |
| E-mail(s) |
fiemed@hotmail.com
|
| Organization name |
HOSPITAL GENERAL DE MEXICO OD
|
| Department |
MEDICINA GENOMICA
|
| Lab |
MEDICINA GENOMICA
|
| Street address |
DR. BALMIS 148
|
| City |
MEXICO CITY |
| State/province |
CUAUHTEMOC |
| ZIP/Postal code |
06726 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL16131 |
| Series (2) |
| GSE52902 |
Impact of Gene Dosage on Gene Expression, Biological Processes and Survival in Cervical Cancer: a Genome-Wide Follow-Up Study [CytoScanHD_Array] |
| GSE52904 |
Impact of Gene Dosage on Gene Expression, Biological Processes and Survival in Cervical Cancer: a Genome-Wide Follow-Up Study |
|
