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Sample GSM1278117 Query DataSets for GSM1278117
Status Public on Oct 15, 2014
Title serum_healthy control_8
Sample type RNA
 
Source name serum
Organism Homo sapiens
Characteristics samples of different individuals pooled?: no
age: 80
gender: male
als mutation: /
Treatment protocol Serum samples were processed within 1 h after blood drawing and stored at -80° C until further usage.
Extracted molecule total RNA
Extraction protocol Total RNA from 8-10 ml of serum were extracted using the QIAzol lysis reagent (Qiagen) according to the manufacturer's instructions. The aqueous phase was collected and total RNA precipitated by addition of 1.4 volumes of isopropanol and overnight incubation at -20°C. Precipitated RNA was redissolved in water and purified a second time using the miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol FlashTagTM Biotin HSR RNA Labeling Kit (Genisphere, LLC, Hatfield, PA, USA) according to the instructions of the manufacturer
 
Hybridization protocol according to the instructions in the FlashTagTM Biotin HSR RNA Labeling Kit
Scan protocol according to Affymetrix protocols using a GeneChip 3000 Scanner
Description control for comparison with sALS
Data processing Affymetrix Expression Console 1.3.1. for normalization (RMA+DBAG; human only); BRB Array tools for statistical analysis
 
Submission date Dec 03, 2013
Last update date Oct 15, 2014
Contact name Karlheinz Holzmann
E-mail(s) karlheinz.holzmann@uni-ulm.de
Organization name Ulm University
Department Medical Faculty
Lab Genomics Core Facility
Street address Helmholtzstreet 8/1
City Ulm
ZIP/Postal code D-89081
Country Germany
 
Platform ID GPL16384
Series (1)
GSE52917 Early pre-symptomatic and progressive signatures of non-coding RNAs in ALS patients and mutation carriers

Data table header descriptions
ID_REF
VALUE RMA normalized, log2

Data table
ID_REF VALUE
14q0_st 1.098512
14qI-1_st 0.8601896
14qI-1_x_st 0.8629587
14qI-2_st 0.7550003
14qI-3_x_st 0.7981034
14qI-4_st 0.7786202
14qI-4_x_st 1.093009
14qI-5_st 0.9362123
14qI-6_st 1.055856
14qI-6_x_st 0.7865678
14qI-7_st 0.8634214
14qI-8_st 0.8760966
14qI-8_x_st 1.091662
14qI-9_x_st 0.9362123
14qII-10_st 1.018378
14qII-10_x_st 0.6061931
14qII-11_st 0.5375994
14qII-11_x_st 1.302206
14qII-12_st 1.29115
14qII-12_x_st 0.9284737

Total number of rows: 5683

Table truncated, full table size 153 Kbytes.




Supplementary file Size Download File type/resource
GSM1278117_Co8.CEL.gz 656.3 Kb (ftp)(http) CEL
Processed data included within Sample table

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