|
| Status |
Public on Oct 15, 2014 |
| Title |
serum_ALS mutation carrier pool 4 |
| Sample type |
RNA |
| |
|
| Source name |
serum
|
| Organism |
Homo sapiens |
| Characteristics |
samples of different individuals pooled?: yes
age: 44, 46, 47
gender: two male, one female
als mutation: all PFN T109M
|
| Treatment protocol |
Serum samples were processed within 1 h after blood drawing and stored at -80° C until further usage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from 8-10 ml of serum were extracted using the QIAzol lysis reagent (Qiagen) according to the manufacturer's instructions. The aqueous phase was collected and total RNA precipitated by addition of 1.4 volumes of isopropanol and overnight incubation at -20°C. Precipitated RNA was redissolved in water and purified a second time using the miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
FlashTagTM Biotin HSR RNA Labeling Kit (Genisphere, LLC, Hatfield, PA, USA) according to the instructions of the manufacturer
|
| |
|
| Hybridization protocol |
according to the instructions in the FlashTagTM Biotin HSR RNA Labeling Kit
|
| Scan protocol |
according to Affymetrix protocols using a GeneChip 3000 Scanner
|
| Data processing |
Affymetrix Expression Console 1.3.1. for normalization (RMA+DBAG; human only); BRB Array tools for statistical analysis
|
| |
|
| Submission date |
Dec 03, 2013 |
| Last update date |
Oct 15, 2014 |
| Contact name |
Karlheinz Holzmann |
| E-mail(s) |
karlheinz.holzmann@uni-ulm.de
|
| Organization name |
Ulm University
|
| Department |
Medical Faculty
|
| Lab |
Genomics Core Facility
|
| Street address |
Helmholtzstreet 8/1
|
| City |
Ulm |
| ZIP/Postal code |
D-89081 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE52917 |
Early pre-symptomatic and progressive signatures of non-coding RNAs in ALS patients and mutation carriers |
|
