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Sample GSM1278143 Query DataSets for GSM1278143
Status Public on Oct 15, 2014
Title serum_ALS mutation carrier_11
Sample type RNA
 
Source name serum
Organism Homo sapiens
Characteristics samples of different individuals pooled?: no
age: 83
gender: female
als mutation: PFN T109M
Treatment protocol Serum samples were processed within 1 h after blood drawing and stored at -80° C until further usage.
Extracted molecule total RNA
Extraction protocol Total RNA from 8-10 ml of serum were extracted using the QIAzol lysis reagent (Qiagen) according to the manufacturer's instructions. The aqueous phase was collected and total RNA precipitated by addition of 1.4 volumes of isopropanol and overnight incubation at -20°C. Precipitated RNA was redissolved in water and purified a second time using the miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol FlashTagTM Biotin HSR RNA Labeling Kit (Genisphere, LLC, Hatfield, PA, USA) according to the instructions of the manufacturer
 
Hybridization protocol according to the instructions in the FlashTagTM Biotin HSR RNA Labeling Kit
Scan protocol according to Affymetrix protocols using a GeneChip 3000 Scanner
Data processing Affymetrix Expression Console 1.3.1. for normalization (RMA+DBAG; human only); BRB Array tools for statistical analysis
 
Submission date Dec 03, 2013
Last update date Oct 15, 2014
Contact name Karlheinz Holzmann
E-mail(s) karlheinz.holzmann@uni-ulm.de
Organization name Ulm University
Department Medical Faculty
Lab Genomics Core Facility
Street address Helmholtzstreet 8/1
City Ulm
ZIP/Postal code D-89081
Country Germany
 
Platform ID GPL16384
Series (1)
GSE52917 Early pre-symptomatic and progressive signatures of non-coding RNAs in ALS patients and mutation carriers

Data table header descriptions
ID_REF
VALUE RMA normalized, log2

Data table
ID_REF VALUE
14q0_st 1.148272
14qI-1_st 1.438181
14qI-1_x_st 1.281867
14qI-2_st 0.7509006
14qI-3_x_st 1.06843
14qI-4_st 0.549657
14qI-4_x_st 0.9619453
14qI-5_st 0.9650555
14qI-6_st 0.7908037
14qI-6_x_st 1.202361
14qI-7_st 0.9135697
14qI-8_st 0.8862357
14qI-8_x_st 0.7701147
14qI-9_x_st 0.8613347
14qII-10_st 1.12131
14qII-10_x_st 0.6904183
14qII-11_st 0.6393114
14qII-11_x_st 0.9797287
14qII-12_st 0.8187338
14qII-12_x_st 1.257257

Total number of rows: 5683

Table truncated, full table size 153 Kbytes.




Supplementary file Size Download File type/resource
GSM1278143_Ca11.CEL.gz 696.6 Kb (ftp)(http) CEL
Processed data included within Sample table

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