|
| Status |
Public on Dec 04, 2013 |
| Title |
Lgr5-Low1 |
| Sample type |
RNA |
| |
|
| Source name |
OSE_low-Lgr5EGFP expression
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
age: 8-12 weeks
genotype/variation: Lgr5-eGFP-CreERT2
cell type: ovarian surface epithelium (OSE) cells
lgr5 expression level: Low (expressing low EGFP)
|
| Treatment protocol |
NONE
|
| Growth protocol |
Female mice of 8-12 weeks age were used for all samples
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Sorted cells were processed in Trizol for RNA extraction as per manufacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared following standard NuGen WT-Ovation Pico RNA Amplification system from 500pg-50 ng total RNA and Nugen Encore Biotin Module
|
| |
|
| Hybridization protocol |
As per manufacturer's protocol, 2.5 ug of biotinylated cDNA were hybridized onto the Affymetrix Mouse GeneChip ST 2.0 arrays for 18 hr at 45C. GeneChips were then washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
Scanning of GeneChips was performed on the Affymetrix GeneChip 7G 3000
|
| Description |
Gene expression data from ovarian surface epithelium cells expressing Low-Lgr5EGFP levels
|
| Data processing |
Expression data were processed using Affymetrix GeneChip Command Console (Affymetrix, Inc) to create raw data (.CEL) files, which were processed and on Affymetrix Expression Console software using default RMA Gene analysis settings (Affymetrix, Inc). Probe summarization (Robust Multichip Analysis, RMA), quality control analysis, and probe annotation were performed according to recommended guidelines (Expression Console Software, Affymetrix, Inc.).
|
| |
|
| Submission date |
Dec 03, 2013 |
| Last update date |
Jul 14, 2014 |
| Contact name |
Nick Barker |
| Organization name |
A*STAR Institute of Medical Biology
|
| Lab |
Adult Stem Cells
|
| Street address |
8A Biomedical Grove, Immunos
|
| City |
Singapore |
| ZIP/Postal code |
238648 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE52925 |
Transcriptome Profiling of Lgr5-expressing ovarian surface epithelium cells |
|
