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| Status |
Public on Sep 29, 2014 |
| Title |
Individual_7 |
| Sample type |
SRA |
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| Source name |
Plasma
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| Organism |
Homo sapiens |
| Characteristics |
tissue: plasma
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| Extracted molecule |
total RNA |
| Extraction protocol |
Blood samples were collected in EDTA-treated tubes and plasma separated immediately by centrifugation for 10 minutes at 1,000g for 10 min and subsequently at 10,000g for 10 min prior to RNA extraction using a miRNeasy kit (Qiagen, Crawley, UK).
Small RNA libraries were prepared using an Illumina Truseq small RNA sample prep kit
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina MiSeq |
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| Data processing |
Basecalls performed using Illumina Real Time Analysis (RTA 1.17.22)
Sequenced reads were trimmed to remove adaptor sequences and collapsed to generate unique reads using Genomics Workbanch v5.5.1 (CLCbio, Aarhus, Denmark)
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| Submission date |
Dec 04, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
David Arthur Simpson |
| E-mail(s) |
david.simpson@qub.ac.uk
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| Organization name |
Queen's University Belfast
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| Department |
Centre for Experimental Medicine
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| Street address |
QUB Institue of clinical Science, Block A, RVH
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| City |
Belfast |
| State/province |
N. Ireland |
| ZIP/Postal code |
BT12 6BA |
| Country |
United Kingdom |
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| Platform ID |
GPL15520 |
| Series (1) |
| GSE52981 |
Small RNA sequencing of plasma RNA |
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| Relations |
| BioSample |
SAMN02435738 |
| SRA |
SRX387062 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM1279746_Sample7_Small_RNAs.txt.gz |
364.5 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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