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Status |
Public on Sep 29, 2014 |
Title |
Individual_8 |
Sample type |
SRA |
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Source name |
Plasma
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Organism |
Homo sapiens |
Characteristics |
tissue: plasma
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Extracted molecule |
total RNA |
Extraction protocol |
Blood samples were collected in EDTA-treated tubes and plasma separated immediately by centrifugation for 10 minutes at 1,000g for 10 min and subsequently at 10,000g for 10 min prior to RNA extraction using a miRNeasy kit (Qiagen, Crawley, UK). Small RNA libraries were prepared using an Illumina Truseq small RNA sample prep kit
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
size fractionation |
Instrument model |
Illumina MiSeq |
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Data processing |
Basecalls performed using Illumina Real Time Analysis (RTA 1.17.22) Sequenced reads were trimmed to remove adaptor sequences and collapsed to generate unique reads using Genomics Workbanch v5.5.1 (CLCbio, Aarhus, Denmark)
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Submission date |
Dec 04, 2013 |
Last update date |
May 15, 2019 |
Contact name |
David Arthur Simpson |
E-mail(s) |
david.simpson@qub.ac.uk
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Organization name |
Queen's University Belfast
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Department |
Centre for Experimental Medicine
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Street address |
QUB Institue of clinical Science, Block A, RVH
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City |
Belfast |
State/province |
N. Ireland |
ZIP/Postal code |
BT12 6BA |
Country |
United Kingdom |
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Platform ID |
GPL15520 |
Series (1) |
GSE52981 |
Small RNA sequencing of plasma RNA |
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Relations |
BioSample |
SAMN02435739 |
SRA |
SRX387063 |
Supplementary file |
Size |
Download |
File type/resource |
GSM1279747_Sample8_Small_RNAs.txt.gz |
474.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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