|
| Status |
Public on Dec 31, 2016 |
| Title |
dmso_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
ZF4 cells, equivalent DMSO
|
| Organism |
Danio rerio |
| Characteristics |
cell line: ZF4
treatment: equivalent DMSO
|
| Treatment protocol |
ZF4 cells were treated with 100nM final concentration of rapamycin (Calbiochem, 553210) and DMSO as control for 48h.
|
| Growth protocol |
ZF4 (ATCC, CRL-2050) were cultured in DMEM/F-12 (1:1) containing 10% fetal bovine serum with 1% Streptomycin/Penicilin =at 28ºC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with SV total RNA isolation kit (Promega). RIN values were calculated using Agilent 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA).
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA was obtained using antisense RNA, 3' IVT Express Kit, (Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
Stained with streptavidin-phycoerythrin conjugate.
|
| Scan protocol |
Scanned with GeneChip scanner.
|
| Description |
DMSO2_ZF4
ZF4 cells treated with equivalent DMSO.
|
| Data processing |
Data was RMA normalized and analyzed with BRB ArrayTools software.
|
| |
|
| Submission date |
Dec 06, 2013 |
| Last update date |
Dec 31, 2016 |
| Contact name |
Ozlen Konu |
| E-mail(s) |
konu@fen.bilkent.edu.tr
|
| Organization name |
Bilkent University
|
| Department |
Molecular Biology and Genetics
|
| Street address |
Bilkent University
|
| City |
Ankara |
| ZIP/Postal code |
06800 |
| Country |
Turkey |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE53086 |
Effects of rapamycin exposure on zebrafish ZF4 cells |
|
