|
| Status |
Public on Dec 10, 2013 |
| Title |
BLIMP1 conditional knockout BMDMs uninfected, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow derived macrophages from BLIMP1 conditional knockout mouse
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Bone marrow derived macrophages
strain: C57BL/6
genotype: BLIMP conditional knockout
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified using the RNeasy mini kit from Qiagen.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized on genechip mouse 430 2.0 genome Array (Affymetrix) . GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
The arrays were scanned using an Affymetrix GeneChip Scanner 3000
|
| Data processing |
Affymetrix CEL files were normalized in Bioconductor using the GCRMA method. Differentially regulated genes were identified using a moderated t-test. False discovery rate adjusted P-values were calculated using the method of Benjamini and Hochberg.
|
| |
|
| Submission date |
Dec 09, 2013 |
| Last update date |
Dec 10, 2013 |
| Contact name |
Daniel R Caffrey |
| Organization name |
UMASS Medical School
|
| Street address |
364 Plantation St
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE53145 |
The transcriptional repressor BLIMP1 curbs host-defenses by suppressing expression of the chemokine CCL8 |
|
