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Sample GSM1283274 Query DataSets for GSM1283274
Status Public on Mar 31, 2015
Title Untreated dendritic cells, biological rep 1 (DC1)
Sample type RNA
 
Source name Untreated dendritic cells
Organism Homo sapiens
Characteristics generation: cells generated in vitro from blood monocytes
cell type: dendritic cells
treatment: untreated
Treatment protocol No treatment prior to extraction
Growth protocol Human dendritic cells (DC) were generated in vitro from CD14+ monocytes purified from peripheral blood from healthy donors and cultured for six days with 100 ng/mL GM-CSF and 10 ng/mL IL-4. After six days of culture, DC were harvested, washed and cultured in the absence or presence of IL-17A, with or without 2ng/mL IFN-gamma. RNA was extracted from untreated DC at day 0 (DC) or DC cultured for 12 days with IL-17A (DC-17), without or with IFN-g (DC-G17).
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16hr at 45°C one Genechip human genome U133 Plus 2.0 Affymetrix arrays.
Scan protocol The arrays were read with a confocal laser (Genechip laser 3000, Affymetrix)
Description DC1
Data processing Expression values and absent/present/marginal calls were calculated using the GCOS v1.4 software (Affymetrix). Absolute expression transcript levels were normalized for each chip by globally scaling all probe sets to a target signal intensity of 500.
 
Submission date Dec 10, 2013
Last update date Mar 31, 2015
Contact name Karene Mahtouk
Organization name CNRS UMR5239
Department LBMC
Lab Dendritic cells and immune plasticity
Street address 165 Chemin du Grand Revoyet : BP12
City Oullins
ZIP/Postal code 69921
Country France
 
Platform ID GPL570
Series (1)
GSE53163 Expression data from human monocyte-derived dendritic cells treated or not with interleukin 17A (IL-17A)

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
1007_s_at 475.5
1053_at 819.4
117_at 522.2
121_at 740.9
1255_g_at 180.4
1294_at 987.4
1316_at 82
1320_at 30.7
1405_i_at 77.9
1431_at 68.8
1438_at 16.7
1487_at 889.8
1494_f_at 86.1
1552256_a_at 14136
1552257_a_at 1387
1552258_at 107.5
1552261_at 107.2
1552263_at 2259
1552264_a_at 2850
1552266_at 15.7

Total number of rows: 54675

Table truncated, full table size 846 Kbytes.




Supplementary file Size Download File type/resource
GSM1283274_DC1.CEL.gz 4.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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