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Sample GSM1283280 Query DataSets for GSM1283280
Status Public on Mar 31, 2015
Title IL-17A-treated dendritic cells, biological rep 3 (DC3-G17)
Sample type RNA
 
Source name IL-17A-treated dendritic cells, in the presence of IFN-g
Organism Homo sapiens
Characteristics generation: cells generated in vitro from blood monocytes
cell type: dendritic cells
treatment: IL-17A and IFN-g
Treatment protocol No treatment prior to extraction
Growth protocol Human dendritic cells (DC) were generated in vitro from CD14+ monocytes purified from peripheral blood from healthy donors and cultured for six days with 100 ng/mL GM-CSF and 10 ng/mL IL-4. After six days of culture, DC were harvested, washed and cultured in the absence or presence of IL-17A, with or without 2ng/mL IFN-gamma. RNA was extracted from untreated DC at day 0 (DC) or DC cultured for 12 days with IL-17A (DC-17), without or with IFN-g (DC-G17).
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16hr at 45°C one Genechip human genome U133 Plus 2.0 Affymetrix arrays.
Scan protocol The arrays were read with a confocal laser (Genechip laser 3000, Affymetrix)
Description DC3-G17
Data processing Expression values and absent/present/marginal calls were calculated using the GCOS v1.4 software (Affymetrix). Absolute expression transcript levels were normalized for each chip by globally scaling all probe sets to a target signal intensity of 500.
 
Submission date Dec 10, 2013
Last update date Mar 31, 2015
Contact name Karene Mahtouk
Organization name CNRS UMR5239
Department LBMC
Lab Dendritic cells and immune plasticity
Street address 165 Chemin du Grand Revoyet : BP12
City Oullins
ZIP/Postal code 69921
Country France
 
Platform ID GPL570
Series (1)
GSE53163 Expression data from human monocyte-derived dendritic cells treated or not with interleukin 17A (IL-17A)

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
1007_s_at 321.6
1053_at 1072
117_at 569.5
121_at 1044
1255_g_at 5.8
1294_at 1201
1316_at 146.7
1320_at 14.2
1405_i_at 1217
1431_at 25.9
1438_at 23
1487_at 922.1
1494_f_at 42.8
1552256_a_at 5368
1552257_a_at 929.6
1552258_at 174.4
1552261_at 20.5
1552263_at 1039
1552264_a_at 897.6
1552266_at 32.4

Total number of rows: 54675

Table truncated, full table size 844 Kbytes.




Supplementary file Size Download File type/resource
GSM1283280_DC3_G17.CEL.gz 4.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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