|
| Status |
Public on Dec 09, 2016 |
| Title |
alveolar macrophage_treated_2 |
| Sample type |
RNA |
| |
|
| Source name |
alveolar macrophage_treated
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6
treatment: antibiotic
cell type: alveolar macrophage
|
| Treatment protocol |
Mice were treated with broad-spectrum antibiotics (ampicillin1 g ml−1, Mediatech; neomycin sulfate 1 g ml−1, Calbiochem; metronidazole 1 g ml−1, Fluka; and vancomycin 0.5 g ml−1, Sigma) in drinking water for 3 weeks.
|
| Growth protocol |
Specific pathogen-free C57Bl/6 mice were purchased from Harlan Sprague-Dawley (Horst, the Netherlands) and housed in a controlled environemt on 12h/12h light/dark cycles.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy kit procedures including DNAse digestion.
|
| Label |
biotin
|
| Label protocol |
standard biotin Ambion labeling - Illumina
|
| |
|
| Hybridization protocol |
standard Illumina protocol
|
| Scan protocol |
standard Illumina protocol
|
| Description |
9377360001_C
cell
|
| Data processing |
NEQC normalized implemented in beadarray package.
|
| |
|
| Submission date |
Dec 10, 2013 |
| Last update date |
Dec 09, 2016 |
| Contact name |
Brendon Scicluna |
| E-mail(s) |
brendon.scicluna@um.edu.mt
|
| Organization name |
University of Malta
|
| Street address |
Msida campus
|
| City |
Msida |
| ZIP/Postal code |
MSD2020 |
| Country |
Malta |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE53174 |
Genome-wide analysis of whole-lung and alveolar macrophages before and after gut microbiome depletion |
|
