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Sample GSM1285958 Query DataSets for GSM1285958
Status Public on Dec 09, 2016
Title alveolar macrophage_treated_5
Sample type RNA
 
Source name alveolar macrophage_treated
Organism Mus musculus
Characteristics strain: C57BL6
treatment: antibiotic
cell type: alveolar macrophage
Treatment protocol Mice were treated with broad-spectrum antibiotics (ampicillin1 g ml−1, Mediatech; neomycin sulfate 1 g ml−1, Calbiochem; metronidazole 1 g ml−1, Fluka; and vancomycin 0.5 g ml−1, Sigma) in drinking water for 3 weeks.
Growth protocol Specific pathogen-free C57Bl/6 mice were purchased from Harlan Sprague-Dawley (Horst, the Netherlands) and housed in a controlled environemt on 12h/12h light/dark cycles.
Extracted molecule total RNA
Extraction protocol Rneasy kit procedures including DNAse digestion.
Label biotin
Label protocol standard biotin Ambion labeling - Illumina
 
Hybridization protocol standard Illumina protocol
Scan protocol standard Illumina protocol
Description 9377360004_A
cell
Data processing NEQC normalized implemented in beadarray package.
 
Submission date Dec 10, 2013
Last update date Dec 09, 2016
Contact name Brendon Paul Scicluna
E-mail(s) b.scicluna@amsterdamumc.nl
Organization name Academic Medical Center
Street address Meibergdreef 9
City Amsterdam
ZIP/Postal code 1105AZ
Country Netherlands
 
Platform ID GPL6885
Series (1)
GSE53174 Genome-wide analysis of whole-lung and alveolar macrophages before and after gut microbiome depletion

Data table header descriptions
ID_REF
VALUE NEQC-normalized signal
detection Pval

Data table
ID_REF VALUE detection Pval
ILMN_1212607 4.505600662 0.752880922
ILMN_1212612 4.490505729 0.782330346
ILMN_1212619 4.957268303 0.176696543
ILMN_1212628 4.61693053 0.510883483
ILMN_1212632 4.744085568 0.325224072
ILMN_1212636 10.45409218 0
ILMN_1212637 9.162441484 0
ILMN_1212645 4.651029564 0.445582586
ILMN_1212648 8.304124428 0
ILMN_1212653 5.942718162 0.003841229
ILMN_1212672 7.890717618 0
ILMN_1212682 4.562883577 0.61971831
ILMN_1212683 4.686050116 0.395646607
ILMN_1212685 5.052581191 0.145966709
ILMN_1212692 5.5470313 0.040973111
ILMN_1212693 6.82096397 0
ILMN_1212695 5.667869812 0.020486556
ILMN_1212698 4.723104207 0.349551857
ILMN_1212702 14.68386931 0
ILMN_1212703 7.031847196 0

Total number of rows: 25681

Table truncated, full table size 823 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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