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Sample GSM1285968 Query DataSets for GSM1285968
Status Public on Dec 09, 2016
Title alveolar macrophage_untreated_7
Sample type RNA
 
Source name alveolar macrophage_untreated
Organism Mus musculus
Characteristics strain: C57BL6
treatment: none
cell type: alveolar macrophage
Treatment protocol Mice were treated with broad-spectrum antibiotics (ampicillin1 g ml−1, Mediatech; neomycin sulfate 1 g ml−1, Calbiochem; metronidazole 1 g ml−1, Fluka; and vancomycin 0.5 g ml−1, Sigma) in drinking water for 3 weeks.
Growth protocol Specific pathogen-free C57Bl/6 mice were purchased from Harlan Sprague-Dawley (Horst, the Netherlands) and housed in a controlled environemt on 12h/12h light/dark cycles.
Extracted molecule total RNA
Extraction protocol Rneasy kit procedures including DNAse digestion.
Label biotin
Label protocol standard biotin Ambion labeling - Illumina
 
Hybridization protocol standard Illumina protocol
Scan protocol standard Illumina protocol
Description 9377360006_C
cell
Data processing NEQC normalized implemented in beadarray package.
 
Submission date Dec 10, 2013
Last update date Dec 09, 2016
Contact name Brendon Paul Scicluna
E-mail(s) b.scicluna@amsterdamumc.nl
Organization name Academic Medical Center
Street address Meibergdreef 9
City Amsterdam
ZIP/Postal code 1105AZ
Country Netherlands
 
Platform ID GPL6885
Series (1)
GSE53174 Genome-wide analysis of whole-lung and alveolar macrophages before and after gut microbiome depletion

Data table header descriptions
ID_REF
VALUE NEQC-normalized signal
detection Pval

Data table
ID_REF VALUE detection Pval
ILMN_1212607 4.552429781 0.565669701
ILMN_1212612 5.83125926 0
ILMN_1212619 5.14105697 0.040312094
ILMN_1212628 4.514881057 0.673602081
ILMN_1212632 4.839916654 0.143042913
ILMN_1212636 10.03249339 0
ILMN_1212637 8.796847204 0
ILMN_1212645 4.630069635 0.377113134
ILMN_1212648 7.692267286 0
ILMN_1212653 8.257596668 0
ILMN_1212672 7.373195985 0
ILMN_1212682 4.57212325 0.512353706
ILMN_1212683 4.438624746 0.873862159
ILMN_1212685 4.426195226 0.902470741
ILMN_1212692 5.98359458 0
ILMN_1212693 7.955557926 0
ILMN_1212695 6.170912772 0
ILMN_1212698 4.40612418 0.945383615
ILMN_1212702 14.68386931 0
ILMN_1212703 6.931504493 0

Total number of rows: 25681

Table truncated, full table size 806 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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