NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1285969 Query DataSets for GSM1285969
Status Public on Dec 09, 2016
Title whole lung_untreated_7
Sample type RNA
 
Source name whole lung_untreated
Organism Mus musculus
Characteristics strain: C57BL6
treatment: none
tissue: whole lung
Treatment protocol Mice were treated with broad-spectrum antibiotics (ampicillin1 g ml−1, Mediatech; neomycin sulfate 1 g ml−1, Calbiochem; metronidazole 1 g ml−1, Fluka; and vancomycin 0.5 g ml−1, Sigma) in drinking water for 3 weeks.
Growth protocol Specific pathogen-free C57Bl/6 mice were purchased from Harlan Sprague-Dawley (Horst, the Netherlands) and housed in a controlled environemt on 12h/12h light/dark cycles.
Extracted molecule total RNA
Extraction protocol Rneasy kit procedures including DNAse digestion.
Label biotin
Label protocol standard biotin Ambion labeling - Illumina
 
Hybridization protocol standard Illumina protocol
Scan protocol standard Illumina protocol
Description 9377360006_D
tissue
Data processing NEQC normalized implemented in beadarray package.
 
Submission date Dec 10, 2013
Last update date Dec 09, 2016
Contact name Brendon Paul Scicluna
E-mail(s) b.scicluna@amsterdamumc.nl
Organization name Academic Medical Center
Street address Meibergdreef 9
City Amsterdam
ZIP/Postal code 1105AZ
Country Netherlands
 
Platform ID GPL6885
Series (1)
GSE53174 Genome-wide analysis of whole-lung and alveolar macrophages before and after gut microbiome depletion

Data table header descriptions
ID_REF
VALUE NEQC-normalized signal
detection Pval

Data table
ID_REF VALUE detection Pval
ILMN_1212607 4.391178855 0.953185956
ILMN_1212612 7.153219112 0
ILMN_1212619 5.152776553 0.00520156
ILMN_1212628 4.579189396 0.43953186
ILMN_1212632 4.824848065 0.11573472
ILMN_1212636 9.965021437 0
ILMN_1212637 9.111993574 0
ILMN_1212645 5.327104252 0
ILMN_1212648 6.462107345 0
ILMN_1212653 9.07902249 0
ILMN_1212672 7.043007716 0
ILMN_1212682 4.683741445 0.250975293
ILMN_1212683 4.728721535 0.187256177
ILMN_1212685 4.482393075 0.717815345
ILMN_1212692 6.737510416 0
ILMN_1212693 8.101670237 0
ILMN_1212695 5.313745156 0
ILMN_1212698 4.41496061 0.905071521
ILMN_1212702 14.48962713 0
ILMN_1212703 6.41807431 0

Total number of rows: 25681

Table truncated, full table size 799 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap