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Sample GSM128919 Query DataSets for GSM128919
Status Public on Sep 01, 2006
Title plusDRB_mouse_COC_cultured_25min_(with_transcriptional_inhibitor)
Sample type SAGE
Anchor NlaIII
Tag Count 36049
Tag Length 10
 
Source name Mouse COC harvested from PMSG-primed females
Organism Mus musculus
Characteristics Mouse strain: Swiss-albino; age: 20-23 days old; pretreatment: PMSG 48 h before ovary harvest and COC collection. COC released from ovaries into DRB-containing medium. DRB is a transcriptional inhibitor.
Treatment protocol Murine COC cultured 25 min in Waymouth medium in atmosphere of 5% CO2 in air in the presence of 120 uM DRB, a transcriptional inhibitor. For protocol reference see:
Rodriguez et al., 2006. Identification of candidate mRNAs associated with gonadotropin-induced maturation of murine cumulus oocyte complexes using serial analysis of gene expression. Physiological Genomics (in press).
Extracted molecule total RNA
Extraction protocol See following reference:
Rodriguez et al., 2006. Identification of candidate mRNAs associated with gonadotropin-induced maturation of murine cumulus oocyte complexes using serial analysis of gene expression. Physiological Genomics (in press).
 
Description plusDRB (treatment) SAGE library used to compare with minusDRB (control) SAGE library. See following reference:
Rodriguez et al., 2006. Identification of candidate mRNAs associated with gonadotropin-induced maturation of murine cumulus oocyte complexes using serial analysis of gene expression. Physiological Genomics (in press).
 
Submission date Aug 15, 2006
Last update date Aug 22, 2006
Contact name Charlotte E. Farin
E-mail(s) Char_Farin@ncsu.edu
Phone 919-515-4022
Fax 919-515-6884
Organization name North Carolina State University
Department Department of Animal Science
Street address 231B Polk Hall
City Raleigh
State/province NC
ZIP/Postal code 28695-7621
Country USA
 
Platform ID GPL11
Series (1)
GSE5568 SAGE in Cultured Murine COC During Early Maturation

Data table header descriptions
TAG Tag_Sequence_plusDRB
COUNT Tag_Count_plusDRB

Data table
TAG COUNT
GTGGCTCACA 996
TGGGTTGTCT 367
GCTGCCCTCC 316
GGCTTCGGTC 315
GATTCCGTGA 292
CACAAACGGT 265
CTAGTCTTTG 264
TGGATCAGTC 244
GCCCGGGAAT 237
ATACTGACAT 236
GGATTTGGCT 210
TGTGCCAAGT 208
CCAGAACAGA 204
GCCTCTGCAT 197
TGTAGTGTAA 195
TGACCCCGGG 193
GCCTTTATGA 192
AGGTCGGGTG 185
CGCCGCCGGC 184
AACAATTTGG 181

Total number of rows: 5024

Table truncated, full table size 64 Kbytes.




Supplementary data files not provided

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