|
Status |
Public on Sep 01, 2006 |
Title |
plusDRB_mouse_COC_cultured_25min_(with_transcriptional_inhibitor) |
Sample type |
SAGE |
Anchor |
NlaIII
|
Tag Count |
36049 |
Tag Length |
10 |
|
|
Source name |
Mouse COC harvested from PMSG-primed females
|
Organism |
Mus musculus |
Characteristics |
Mouse strain: Swiss-albino; age: 20-23 days old; pretreatment: PMSG 48 h before ovary harvest and COC collection. COC released from ovaries into DRB-containing medium. DRB is a transcriptional inhibitor.
|
Treatment protocol |
Murine COC cultured 25 min in Waymouth medium in atmosphere of 5% CO2 in air in the presence of 120 uM DRB, a transcriptional inhibitor. For protocol reference see: Rodriguez et al., 2006. Identification of candidate mRNAs associated with gonadotropin-induced maturation of murine cumulus oocyte complexes using serial analysis of gene expression. Physiological Genomics (in press).
|
Extracted molecule |
total RNA |
Extraction protocol |
See following reference: Rodriguez et al., 2006. Identification of candidate mRNAs associated with gonadotropin-induced maturation of murine cumulus oocyte complexes using serial analysis of gene expression. Physiological Genomics (in press).
|
|
|
Description |
plusDRB (treatment) SAGE library used to compare with minusDRB (control) SAGE library. See following reference: Rodriguez et al., 2006. Identification of candidate mRNAs associated with gonadotropin-induced maturation of murine cumulus oocyte complexes using serial analysis of gene expression. Physiological Genomics (in press).
|
|
|
Submission date |
Aug 15, 2006 |
Last update date |
Aug 22, 2006 |
Contact name |
Charlotte E. Farin |
E-mail(s) |
Char_Farin@ncsu.edu
|
Phone |
919-515-4022
|
Fax |
919-515-6884
|
Organization name |
North Carolina State University
|
Department |
Department of Animal Science
|
Street address |
231B Polk Hall
|
City |
Raleigh |
State/province |
NC |
ZIP/Postal code |
28695-7621 |
Country |
USA |
|
|
Platform ID |
GPL11 |
Series (1) |
GSE5568 |
SAGE in Cultured Murine COC During Early Maturation |
|