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Status
Public on Jun 13, 2008
Title
PBS_6HRS_rep02
Sample type
RNA
Source name
pbs challenge 6hrs
Organism
Mus musculus Characteristics
'strain:BALB/c WT', 'gender:male', 'age:8wks', 'tissue: whole lung', 'condition: pbs challenge 6hrs'
Extracted molecule
total RNA
Extraction protocol
trizol extraction followed by qiagen RNeasy total RNA cleanup protocol
Label
biotin
Label protocol
standard Affymetrix procedures
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures using affy GCS3000
Description
Intensity of hybridization for each probe pair was computed by GCOS 1.2 software (Affymetrix). (For more detailed methods, please refer to the website of the Malaria Research Institute Gene Array Core Facility (MRI-GACF) at the Johns Hopkins Bloomberg School of Public Health http://jhmmi.jhsph.edu )
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Aug 16, 2006
Last update date
Aug 28, 2018
Contact name
Joshua J Reece
E-mail(s)
josh.reece@gmail.com Organization name
Johns Hopkins University BSPH
Department
MMI
Lab
Lab of Alan Scott
Street address
615 N. Wolfe Street
City
Baltimore
State/province
MD
ZIP/Postal code
21205
Country
USA
Platform ID
GPL1261 Series (1)
GSE5555 Hookworm-Induced Persistent Changes to the Immunological Environment of the Lung
Relations Reanalyzed by
GSE119085
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
Data table ID_REF VALUE ABS_CALL AFFX-BioB-5_at
553.9
P
AFFX-BioB-M_at
783.4
P
AFFX-BioB-3_at
370.5
P
AFFX-BioC-5_at
1836.2
P
AFFX-BioC-3_at
2038.9
P
AFFX-BioDn-5_at
2591.8
P
AFFX-BioDn-3_at
8786.7
P
AFFX-CreX-5_at
14882.4
P
AFFX-CreX-3_at
20970
P
AFFX-DapX-5_at
5.2
A
AFFX-DapX-M_at
12.4
A
AFFX-DapX-3_at
16.8
A
AFFX-LysX-5_at
16
A
AFFX-LysX-M_at
11.1
A
AFFX-LysX-3_at
17.3
A
AFFX-PheX-5_at
6.6
A
AFFX-PheX-M_at
5.3
A
AFFX-PheX-3_at
54.4
A
AFFX-ThrX-5_at
32.8
A
AFFX-ThrX-M_at
12
A
45101 832 Kbytes .Supplementary file Size Download File type/resource GSM129101.CEL.gz
5.9 Mb
(ftp) (http) CEL
