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Status
Public on Jun 13, 2008
Title
HDM_72HRS_rep02
Sample type
RNA
Source name
hdm challenge 72hrs
Organism
Mus musculus Characteristics
'strain:BALB/c WT', 'gender:male', 'age:8wks', 'tissue: whole lung', 'condition: hdm challenge 72hrs'
Extracted molecule
total RNA
Extraction protocol
trizol extraction followed by qiagen RNeasy total RNA cleanup protocol
Label
biotin
Label protocol
standard Affymetrix procedures
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures using affy GCS3000
Description
Intensity of hybridization for each probe pair was computed by GCOS 1.2 software (Affymetrix). (For more detailed methods, please refer to the website of the Malaria Research Institute Gene Array Core Facility (MRI-GACF) at the Johns Hopkins Bloomberg School of Public Health http://jhmmi.jhsph.edu )
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Aug 16, 2006
Last update date
Aug 28, 2018
Contact name
Joshua J Reece
E-mail(s)
josh.reece@gmail.com Organization name
Johns Hopkins University BSPH
Department
MMI
Lab
Lab of Alan Scott
Street address
615 N. Wolfe Street
City
Baltimore
State/province
MD
ZIP/Postal code
21205
Country
USA
Platform ID
GPL1261 Series (1)
GSE5555 Hookworm-Induced Persistent Changes to the Immunological Environment of the Lung
Relations Reanalyzed by
GSE119085
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
Data table ID_REF VALUE ABS_CALL AFFX-BioB-5_at
548.7
P
AFFX-BioB-M_at
894.6
P
AFFX-BioB-3_at
519.9
P
AFFX-BioC-5_at
2179.5
P
AFFX-BioC-3_at
2054.6
P
AFFX-BioDn-5_at
2734.1
P
AFFX-BioDn-3_at
10132.1
P
AFFX-CreX-5_at
18452.1
P
AFFX-CreX-3_at
24833.2
P
AFFX-DapX-5_at
52.5
A
AFFX-DapX-M_at
40
A
AFFX-DapX-3_at
9.8
A
AFFX-LysX-5_at
22.6
A
AFFX-LysX-M_at
36.9
A
AFFX-LysX-3_at
26.5
A
AFFX-PheX-5_at
6.6
A
AFFX-PheX-M_at
4.8
A
AFFX-PheX-3_at
20.7
A
AFFX-ThrX-5_at
13.8
A
AFFX-ThrX-M_at
13.7
A
45101 833 Kbytes .Supplementary file Size Download File type/resource GSM129128.CEL.gz
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(ftp) (http) CEL
