|
| Status |
Public on Feb 19, 2014 |
| Title |
Brain-Young-Male-2 |
| Sample type |
RNA |
| |
|
| Source name |
young zebrafish, brain, male
|
| Organism |
Danio rerio |
| Characteristics |
tissue: brain
strain: AB
|
| Treatment protocol |
For the brain dissection, fish were anesthetized in Tricaine (Sigma-Aldrich, Germany) and head was separated from the body with a scalpel. The eyes/optic nerves were dissected, brain was dissected away from dorsal surface. Following visualization of the brain, it was removed from the skull. Tissues were weighed in 0.5 ml microtubes, frozen in liquid nitrogen and stored at -80°C until RNA isolation.
|
| Growth protocol |
Fish were maintained at a constant temperature of 28°C on a 14L:10D light cycle. All the fish were fed three times daily and kept in a recirculating system at all times. All animals were kept in groups, around 10 animals in a 4L tank.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation was performed with a RNAeasy Mini Kit (Qiagen, Germany). DNase treatment was done with RNase-free DNase Set (Qiagen, Germany).
|
| Label |
Biotin
|
| Label protocol |
RNA was amplified with Ovation RNA Amplification System (NuGen) and biotin-labeled with Encore Biotin Module (NuGen).
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
Affymetrix protocol
|
| Description |
7.5 month old male, fish no: HD82
|
| Data processing |
Raw Affymetrix CEL files were processed with RMA normalization method. Probesets were annotated using BioMart (http://www.biomart.org/biomart/martview) with the current Zebrafish Genome Built (Danio rerio Zv9).
|
| |
|
| Submission date |
Dec 18, 2013 |
| Last update date |
Feb 19, 2014 |
| Contact name |
Ayca Arslan Ergul |
| E-mail(s) |
aycaergul@gmail.com
|
| Organization name |
Bilkent University
|
| Department |
UNAM
|
| Street address |
Bilkent Üniversitesi UNAM
|
| City |
ANKARA |
| State/province |
ÇANKAYA |
| ZIP/Postal code |
06800 |
| Country |
Turkey |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE53430 |
Expression data from young and old zebrafish brain. |
|
