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Sample GSM1293440 Query DataSets for GSM1293440
Status Public on May 31, 2015
Title aorta of EC-Dicerflox mice Replicate 3
Sample type RNA
Source name aorta, HFD for 4 weeks
Organism Mus musculus
Characteristics strain: VE-Cadherin-CreERT2Dicer1flox/flox/Apoe-/-
age: 6-8 weeks
gender: male
protocol: HFD for 4 weeks
tissue: aorta
Extracted molecule total RNA
Extraction protocol mirVana miRNA Isolation Kit, see
Label FAM
Label protocol N/A
Hybridization protocol N/A
Scan protocol N/A
Description Biological replicate 3 of 3. (Dicer knockout group
EC-Dicerflox 3
Data processing Taqman low density arrays were run on the 7900 HT Sequence Detection system. The ABI TaqMan SDS v2.3 software was utilized to obtain raw CT values. To review results, the raw CT data (SDS file format) were exported from the Plate Centric View into the ABI TaqMan RQ manager software. Automatic baseline and manual CTs were calculated with the RQ Manager Software for all samples. Statistical analysis: The global data set of miRs from card A and B was used for analysis. Data were calculated using Real Time StatMiner Tools [Integromics.]. Input data for analysis consisted of all samples. CT values were normalized to the most stable endogenous controls (snoRNA202, snoRNA135, snoRNA429 and MammU6 for Card A and B) according to the Genorm algorithm.
Submission date Dec 18, 2013
Last update date May 31, 2015
Contact name Andreas Schober
Organization name Ludwig-Maximilians-University
Department Institute for Cardiovascular Prevention
Street address Pettenkoferstr. 9
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
Platform ID GPL18082
Series (1)
GSE53435 MiRNA expression profile in the aortas of Apoe-/- mice with an specific knock-out of Dicer in endothelial cells compared to control mice after 4 weeks of a high-fat diet

Data table header descriptions
VALUE [delta] CT = CT,sample - CT,endogenous control.

Data table
ath-miR159a-4373390 21.375
mmu-let-7a*-002478 6.029
mmu-let-7a-4373169 3.360
mmu-let-7b-4373168 3.664
mmu-let-7c-1*-002479 8.448
mmu-let-7c-4373167 2.384
mmu-let-7d*-001178 18.480
mmu-let-7d-4395394 3.562
mmu-let-7e-4395517 2.251
mmu-let-7f-4373164 5.170
mmu-let-7g*-002492 10.463
mmu-let-7g-4395393 4.356
mmu-let-7i-4395332 3.551
mmu-miR-100-4373160 14.567
mmu-miR-101a*-002507 8.475
mmu-miR-101a-4395364 7.016
mmu-miR-101b-002531 4.441
mmu-miR-103-4373158 6.353
mmu-miR-105-4395595 21.375
mmu-miR-106a-4395589 3.948

Total number of rows: 599

Table truncated, full table size 15 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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