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Sample GSM1293443 Query DataSets for GSM1293443
Status Public on May 31, 2015
Title aorta of EC-DicerWT mice Replicate 3
Sample type RNA
 
Source name aorta, HFD for 4 weeks
Organism Mus musculus
Characteristics strain: VE-Cadherin-CreERT2Dicer1WT/WT/Apoe-/-
age: 6-8 weeks
gender: male
protocol: HFD for 4 weeks
tissue: aorta
Extracted molecule total RNA
Extraction protocol mirVana miRNA Isolation Kit, see http://www.ambion.com/catalog/CatNum.php?1560
Label FAM
Label protocol N/A
 
Hybridization protocol N/A
Scan protocol N/A
Description Biological replicate 3 of 3. (Dicer wildtype group)
EC-DicerWT 3
Data processing Taqman low density arrays were run on the 7900 HT Sequence Detection system. The ABI TaqMan SDS v2.3 software was utilized to obtain raw CT values. To review results, the raw CT data (SDS file format) were exported from the Plate Centric View into the ABI TaqMan RQ manager software. Automatic baseline and manual CTs were calculated with the RQ Manager Software for all samples. Statistical analysis: The global data set of miRs from card A and B was used for analysis. Data were calculated using Real Time StatMiner Tools [Integromics. http://www.integromics.com/StatMiner.php]. Input data for analysis consisted of all samples. CT values were normalized to the most stable endogenous controls (snoRNA202, snoRNA135, snoRNA429 and MammU6 for Card A and B) according to the Genorm algorithm.
 
Submission date Dec 18, 2013
Last update date May 31, 2015
Contact name Andreas Schober
E-mail(s) aschober@med.lmu.de
Organization name Ludwig-Maximilians-University
Department Institute for Cardiovascular Prevention
Street address Pettenkoferstr. 9
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
 
Platform ID GPL18082
Series (1)
GSE53435 MiRNA expression profile in the aortas of Apoe-/- mice with an specific knock-out of Dicer in endothelial cells compared to control mice after 4 weeks of a high-fat diet

Data table header descriptions
ID_REF
VALUE [delta] CT = CT,sample - CT,endogenous control.

Data table
ID_REF VALUE
ath-miR159a-4373390 21.619
mmu-let-7a*-002478 5.295
mmu-let-7a-4373169 3.541
mmu-let-7b-4373168 3.888
mmu-let-7c-1*-002479 8.722
mmu-let-7c-4373167 2.400
mmu-let-7d*-001178 19.312
mmu-let-7d-4395394 3.536
mmu-let-7e-4395517 2.407
mmu-let-7f-4373164 4.894
mmu-let-7g*-002492 9.872
mmu-let-7g-4395393 4.097
mmu-let-7i-4395332 3.375
mmu-miR-100-4373160 21.619
mmu-miR-101a*-002507 9.316
mmu-miR-101a-4395364 6.914
mmu-miR-101b-002531 4.529
mmu-miR-103-4373158 6.004
mmu-miR-105-4395595 21.619
mmu-miR-106a-4395589 3.972

Total number of rows: 599

Table truncated, full table size 15 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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