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Sample GSM1294948 Query DataSets for GSM1294948
Status Public on Dec 17, 2016
Title Aph-IVF96 rep1
Sample type RNA
 
Source name Aph-IVF96 rep1
Organism Mus musculus
Characteristics aphidicolin treated: No
treatment: none
sample type: in vitro fertilization (96h)
cell type: blastocysts
Extracted molecule total RNA
Extraction protocol 150 ng of total DNA-free RNA was extrated using ZR RNA MicroPrep kit (ZYMO RESEARCH, R1061) following the manufacturer's instructions (IVT: 17h)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina),
background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model,
variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Dec 19, 2013
Last update date Jan 15, 2022
Contact name Marcos J. Araúzo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Phone +34 943 00 6108
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bionformatics
Street address Rogentstrasse
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE53497 First round of DNA replication is essential for transcriptional reprogramming of somatic nuclei in mouse oocytes

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 0.564808
ILMN_1212612 -0.144236
ILMN_1212619 0.800894
ILMN_1212628 0.649363
ILMN_1212632 0.985921
ILMN_1212636 8.479620
ILMN_1212637 6.521741
ILMN_1212645 7.368346
ILMN_1212648 4.216484
ILMN_1212653 2.243832
ILMN_1212672 7.009666
ILMN_1212682 1.492211
ILMN_1212683 3.343081
ILMN_1212685 0.612832
ILMN_1212692 2.803274
ILMN_1212693 0.371948
ILMN_1212695 0.423139
ILMN_1212698 0.925170
ILMN_1212702 2.625051
ILMN_1212703 2.361358

Total number of rows: 25697

Table truncated, full table size 552 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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