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Status |
Public on Dec 17, 2016 |
Title |
Aph-IVF96 rep1 |
Sample type |
RNA |
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|
Source name |
Aph-IVF96 rep1
|
Organism |
Mus musculus |
Characteristics |
aphidicolin treated: No treatment: none sample type: in vitro fertilization (96h) cell type: blastocysts
|
Extracted molecule |
total RNA |
Extraction protocol |
150 ng of total DNA-free RNA was extrated using ZR RNA MicroPrep kit (ZYMO RESEARCH, R1061) following the manufacturer's instructions (IVT: 17h)
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
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|
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Hybridization protocol |
Standard Illumina hybridization protocol
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Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 1
|
Data processing |
The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
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|
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Submission date |
Dec 19, 2013 |
Last update date |
Jan 15, 2022 |
Contact name |
Marcos J. Araúzo-Bravo |
E-mail(s) |
mararabra@yahoo.co.uk
|
Phone |
+34 943 00 6108
|
Organization name |
Max Planck Institute for Molecular Biomedicine
|
Department |
Cell and Developmental Biology
|
Lab |
Computational Biology and Bionformatics
|
Street address |
Rogentstrasse
|
City |
Muenster |
ZIP/Postal code |
48149 |
Country |
Germany |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE53497 |
First round of DNA replication is essential for transcriptional reprogramming of somatic nuclei in mouse oocytes |
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