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Sample GSM1296067 Query DataSets for GSM1296067
Status Public on Mar 31, 2014
Title H_lesional_day43
Sample type RNA
 
Source name Lesional skin biopsy from psoriasis patient, 350mg brodalumab treated, day 43
Organism Homo sapiens
Characteristics disease state: psoriasis
tissue: lesional skin
treatment: 350mg brodalumab
time: day 43
patient: H
Extracted molecule total RNA
Extraction protocol The frozen biopsy was homogenized using a TissueRuptor, and RNA was extracted using the mirVana™ kit with on-column DNase treatment.
Label biotin
Label protocol Total RNA was reverse-transcribed and amplified using the NuGen Ovation™ RNA amplification system V2 with Ovation™WB reagent. Amplified RNA was fragmented and labeled using the NuGen FL-Ovation™ cDNA Biotin Module V2. Amplification and labeling were performed on a Beckman-Coulter ArrayPlex.
 
Hybridization protocol Procedure used standard Affymetrix protocol.
Scan protocol Procedure used standard Affymetrix protocol.
Description skn55816
Data processing Scans were imported into Rosetta Resolver and pre-processed using the 'Resolver Affymetrix Rosetta Intensity Profile Builder (saves reporters)' pipeline.
 
Submission date Dec 20, 2013
Last update date Mar 31, 2014
Contact name Jeannette Bigler
E-mail(s) jeannette.bigler@bjgroupllc.com
Organization name BJ Group, LLC
Street address 3200 NE 92nd Street
City Seattle
State/province WA
ZIP/Postal code 98115
Country USA
 
Platform ID GPL570
Series (1)
GSE53552 Gene expression profiling in psoriatic lesional and non-lesional skin [brodalumab treatment]

Data table header descriptions
ID_REF
VALUE Signal intensity normalized using the Resolver 'Resolver Affymetrix Rosetta Intensity Profile Builder (saves reporters)' pipeline and scaled
Detection p-value

Data table
ID_REF VALUE Detection p-value
1007_s_at 2641.31 0.00
1053_at 542.19 0.00
117_at 554.75 0.00
121_at 94.49 0.00
1255_g_at 6.59 0.28
1294_at 613.76 0.00
1316_at 924.60 0.00
1320_at 47.55 0.00
1405_i_at 300.47 0.00
1431_at 225.62 0.00
1438_at 77.04 0.00
1487_at 914.68 0.00
1494_f_at 4.94 0.35
1552256_a_at 461.57 0.00
1552257_a_at 1431.99 0.00
1552258_at 144.95 0.00
1552261_at 9.40 0.29
1552263_at 185.85 0.00
1552264_a_at 200.21 0.00
1552266_at 49.76 0.00

Total number of rows: 54675

Table truncated, full table size 1202 Kbytes.




Supplementary file Size Download File type/resource
GSM1296067_skn55816.CEL.gz 5.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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