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Sample GSM1296477 Query DataSets for GSM1296477
Status Public on Aug 01, 2014
Title Langerin-CD11b- migratory DC rep4
Sample type RNA
 
Source name skin draining LN
Organism Mus musculus
Characteristics strain: C57BL/6
age: 8-12 weeks
injected with: human recombinant Flt3L with 12-14 days (intraperitoneal)
cell subtype: Langerin-CD11b- migratory DC
Growth protocol C57BL/6 mice were injected for 12-14 days with human recombinant Flt3L (Celldex), skin draining LNs (inguinal , axillary, brachial, popliteal and cervical were pooled and DC were preapred by standard collagenase D isolation technique. For some samples mice were treated with 5ug of LPS IV and skin draining LN were harvested 24 hours later. Peritoneal macrophages were isolated from untreated mice
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Purity analysis was performed by nanodrop and Eukaryote Total RNA Pico Series II (Agilent)
Label biotin
Label protocol RNA was amplified and hybridized on the Illumina MouseRef-8 v2.0 Expression BeadChip
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description SAMPLE 13
Data processing The data were normalized using quantile normalization in Genespring v12.5 (Agilent).
 
Submission date Dec 23, 2013
Last update date Aug 01, 2014
Contact name Niroshana Anandasabapathy
Organization name Brigham and Women’s Hospital
Department Department of Dermatology
Street address 221 Longwood Ave. EBRC 513
City Boston
State/province MA
ZIP/Postal code 02143
Country USA
 
Platform ID GPL6885
Series (1)
GSE53588 Analysis of classical and migratory DC from the skin draining LNs of Flt3L treated mice and comparison to LPS treated DC from skin draining LN and peritoneal macrophages

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 0.21947193 0.000232978
ILMN_3122480 -0.07842398 0.025031654
ILMN_2599935 -0.97363186 1.12589E-05
ILMN_2675543 -0.039145947 0.7627606
ILMN_2686883 -0.34393454 0.000356454
ILMN_2751818 0.029817104 0.10153842
ILMN_2728634 0.026521683 0.34594712
ILMN_3040515 -0.09323931 0.31286624
ILMN_2711608 -0.03989029 0.46104372
ILMN_1232875 -0.0284729 0.00397297
ILMN_1258507 -0.45027542 0.003259605
ILMN_2746142 -0.1119771 0.33772096
ILMN_1252690 -0.06845379 0.08506784
ILMN_2655499 0.031309128 2.86115E-05
ILMN_1252870 -0.09835863 0.019852648
ILMN_1248179 0.14341593 0.025250003
ILMN_2649955 -0.13992405 0.002527121
ILMN_2628708 -0.27281237 2.84063E-05
ILMN_3024781 0.26828337 0.056540787
ILMN_2705628 -0.13788414 2.09817E-05

Total number of rows: 25697

Table truncated, full table size 892 Kbytes.




Supplementary file Size Download File type/resource
GSM1296477_SAMPLE_13.csv.gz 384.3 Kb (ftp)(http) CSV
Raw data provided as supplementary file
Processed data included within Sample table
Raw data are available on Series record

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