|
| Status |
Public on Dec 24, 2013 |
| Title |
III-7_Unaffected_Skin |
| Sample type |
RNA |
| |
|
| Source name |
Skin biopsy from III-7, Unaffected
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: skin
source: unaffected skin from III-7
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with miRNeasy kit (Qiagen, Germantown, MD according to the manufacturer's instructions. The quality and quantity of the RNA samples were evaluated by NanoDrop ND-1000 Spectrophotometer (Wilmington, DE), and Agilent Bioanalyzer 2100 (Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
Total RNA was reverse transcribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labeled with cyanine 3-CTP.
|
| |
|
| Hybridization protocol |
The labeled antisense cRNAs were hybridized to microarrays at 65°C for 17h.
|
| Scan protocol |
The arrays were washed and scanned using an Agilent scanner.
|
| Description |
unaffected skin from III-7
|
| Data processing |
The scanned images of the arrays were converted and imported into the Institute for Systems Biology’s Systems Biology Experiment Analysis System (SBEAMS) for array data normalization and further analyses.
|
| |
|
| Submission date |
Dec 23, 2013 |
| Last update date |
Dec 24, 2013 |
| Contact name |
Ji-Hoon Cho |
| E-mail(s) |
jcho@systemsbiology.org
|
| Organization name |
Institute for Systems Biology
|
| Street address |
401 Terry Avenue North
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL17077 |
| Series (2) |
| GSE53613 |
Gene expression signatures in the skin of focal palmoplantar keratoderma (FPPK) patients |
| GSE53616 |
Gene expression signatures in the skin of focal palmoplantar keratoderma (FPPK) patients, and cell lines transfected with mutant TRPV3 |
|
