|
| Status |
Public on Dec 24, 2013 |
| Title |
HaCaT_16hr_muTRPV3 |
| Sample type |
RNA |
| |
|
| Source name |
HaCaT, transfected with mutant TRPV3, 16hr
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HaCaT
transfection: mutant TRPV3
time: 16 hrs
|
| Treatment protocol |
For TRPV3 mutant overexpression, HaCaT cells were transfected in 6-well dishes at approximately 70% confluence using Lipofectamine 2000 (Life Technologies, Carlsbad, CA) according to the manufacturer’s instructions. Control cells were treated with the same plasmid without insert.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with miRNeasy kit (Qiagen, Germantown, MD according to the manufacturer's instructions. The quality and quantity of the RNA samples were evaluated by NanoDrop ND-1000 Spectrophotometer (Wilmington, DE), and Agilent Bioanalyzer 2100 (Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
Total RNA was reverse transcribed and followed by generation of cDNAs. The resulting cDNAs were transcribed and labeled with cyanine 3-CTP.
|
| |
|
| Hybridization protocol |
The labeled antisense cRNAs were hybridized to microarrays at 65°C for 17h.
|
| Scan protocol |
The arrays were washed and scanned using an Agilent scanner.
|
| Description |
Gene expression of HaCaT cell lines at 16hr, transfected with mutant TRPV3
|
| Data processing |
The scanned images of the arrays were converted and imported into the Institute for Systems Biology’s Systems Biology Experiment Analysis System (SBEAMS) for array data normalization and further analyses.
|
| |
|
| Submission date |
Dec 23, 2013 |
| Last update date |
Dec 24, 2013 |
| Contact name |
Ji-Hoon Cho |
| E-mail(s) |
jcho@systemsbiology.org
|
| Organization name |
Institute for Systems Biology
|
| Street address |
401 Terry Avenue North
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL17077 |
| Series (2) |
| GSE53615 |
Gene expression signatures of HaCaT cell lines transfected with mutant TRPV3 |
| GSE53616 |
Gene expression signatures in the skin of focal palmoplantar keratoderma (FPPK) patients, and cell lines transfected with mutant TRPV3 |
|
