|
| Status |
Public on Dec 25, 2013 |
| Title |
EOL1_IMA2 |
| Sample type |
RNA |
| |
|
| Source name |
EOL1 Cell line_IMA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: EOL1
cell type: eosinophilic leukemia cells
tumor type: FIP1L1/PDGFRA+ hypereosinofilic leukemia
treated with: 1 microM imatinib mesylate for 6 hrs
|
| Treatment protocol |
Treatment with imatinib mesylate (0 and 1 microM) for 6 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using the TRIzol reagent (Invitrogen and Life Technologies), purified using the RNeasy Kit (QIAGEN)
|
| Label |
biotin
|
| Label protocol |
Standard HG-U133 2.0 Plus microarrays (Affymetrix, Inc.) label protocol
|
| |
|
| Hybridization protocol |
Standard HG-U133 2.0 Plus microarrays (Affymetrix, Inc.) Hybridization protocol
|
| Scan protocol |
Standard HG-U133 2.0 Plus microarrays (Affymetrix, Inc.) scan protocol
|
| Description |
20061110_Ea1-IMA2_U133Plus
|
| Data processing |
The gene expression values were determined by MAS 5 algorithm in Expression Console (Affymetrix) and normalization was performed by scaling to a target intensity of 500. Normalization quality control was performed by box-plot and MA plot consistency.
|
| |
|
| Submission date |
Dec 24, 2013 |
| Last update date |
Dec 25, 2013 |
| Contact name |
Fabio Fuligni |
| E-mail(s) |
fabio.fuligni2@unibo.it
|
| Organization name |
University of Bologna
|
| Department |
Department of Experimental, Diagnostic, and Specialty Medicine Bologna University Medical School
|
| Lab |
Unit of Hematopathology
|
| Street address |
Via Massarenti 9
|
| City |
Bologna |
| ZIP/Postal code |
40138 |
| Country |
Italy |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE53633 |
Platelet-derived growth factor alpha mediates the proliferation of peripheral T-cell lymphoma cells via an autocrine regulatory pathway |
|
