|
| Status |
Public on Dec 30, 2013 |
| Title |
EPC_acLDL rep1 |
| Sample type |
RNA |
| |
|
| Source name |
EPC_acLDL
|
| Organism |
Mus musculus |
| Characteristics |
strain: athymic nude
tissue: lung
cell type: Endothelial Progenitor Cell (EPC)
identifier: acLDL + staining
passage: passage 3-6
|
| Treatment protocol |
EPC_acLDL cells were labeled with Flourescent acLDL then sorted and only positive cells replated
|
| Growth protocol |
Primary EPC harvested passage 3-6, EPC_acLDL cells harvest passage 3-6, EPC_late harvested passage 16-20; grown in Mouse Endothelial Medium w/20% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
C; Sample C
8924929066_C.AVG_Signal
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Dec 27, 2013 |
| Last update date |
Dec 30, 2013 |
| Contact name |
Jeff Russell |
| E-mail(s) |
russelljs73@gmail.com
|
| Phone |
813-745-8118
|
| Organization name |
Moffitt Cancer Center
|
| Department |
Head and Neck/Endocrine
|
| Street address |
12902 Magnolia Drive
|
| City |
Tampa |
| State/province |
FL |
| ZIP/Postal code |
33612 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE53681 |
Mouse Endothelial Progenitor Cell (EPC) Lines - Illumnia Array |
|
