|
| Status |
Public on Jan 15, 2015 |
| Title |
post-Tx biopsy 02, biopsy pair 1047-02, AKI group |
| Sample type |
RNA |
| |
|
| Source name |
kidney allograft biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: kidney
biopsy type: post-TX biopsy, acute tubular necrosis (ATN)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Kidney tissue in RNAlater, stored at -80°C. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA (1µg per sample) was labeled using the Affymetrix® FlashTag™ Biotin HSR RNA Labeling Kit and hybridized to the arrays as described by the manufacturer (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
The complementary RNA hybridization cocktail was incubated overnight at 48°C while rotating in a hybridization oven. After 16 hours of hybridization, the cocktail was removed and the arrays were washed and stained in an Affymetrix GeneChip fluidics station 450.
|
| Scan protocol |
Arrays were scanned on an Affymetrix GeneChip scanner 7G.
|
| Description |
microRNA expression data from post-TX biopsy, acute tubular necrosis (ATN)
|
| Data processing |
Normalized expression signals were calculated from Affymetrix CEL files using RMA.
|
| |
|
| Submission date |
Jan 02, 2014 |
| Last update date |
Jan 15, 2015 |
| Contact name |
Julia Wilflingseder |
| E-mail(s) |
julia.wilflingseder@meduniwien.ac.at
|
| Organization name |
Medical University of Vienna
|
| Department |
Nephrology and Dialysis
|
| Lab |
Laboratory Rainer Oberbauer
|
| Street address |
Währinger Gürtel 18-20
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL16384 |
| Series (2) |
| GSE53771 |
Molecular regulation of acute kidney injury (miRNA) |
| GSE53773 |
Molecular regulation of acute kidney injury |
|
