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Sample GSM1300460 Query DataSets for GSM1300460
Status Public on Jan 15, 2015
Title post-Tx biopsy 41, biopsy pair 1171-41, PBx group (control)
Sample type RNA
 
Source name kidney allograft biopsy
Organism Homo sapiens
Characteristics tissue: kidney
biopsy type: post-TX biopsy, protocol biopsy (PBx) without pathology
Extracted molecule total RNA
Extraction protocol Kidney tissue in RNAlater, stored at -80°C. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA (1µg per sample) was labeled using the Affymetrix® FlashTag™ Biotin HSR RNA Labeling Kit and hybridized to the arrays as described by the manufacturer (Affymetrix, Santa Clara, CA).
 
Hybridization protocol The complementary RNA hybridization cocktail was incubated overnight at 48°C while rotating in a hybridization oven. After 16 hours of hybridization, the cocktail was removed and the arrays were washed and stained in an Affymetrix GeneChip fluidics station 450.
Scan protocol Arrays were scanned on an Affymetrix GeneChip scanner 7G.
Description microRNA expression data from post-TX biopsy, protocol biopsy (PBx) without pathology
Data processing Normalized expression signals were calculated from Affymetrix CEL files using RMA.
 
Submission date Jan 02, 2014
Last update date Jan 15, 2015
Contact name Julia Wilflingseder
E-mail(s) julia.wilflingseder@meduniwien.ac.at
Organization name Medical University of Vienna
Department Nephrology and Dialysis
Lab Laboratory Rainer Oberbauer
Street address Währinger Gürtel 18-20
City Vienna
ZIP/Postal code 1090
Country Austria
 
Platform ID GPL16384
Series (2)
GSE53771 Molecular regulation of acute kidney injury (miRNA)
GSE53773 Molecular regulation of acute kidney injury

Data table header descriptions
ID_REF
VALUE log2 intensity (RMA)

Data table
ID_REF VALUE
14q0_st 5.168382
14qI-1_st 1.595721
14qI-1_x_st 1.221386
14qI-2_st 1.288009
14qI-3_x_st 3.136668
14qI-4_st 2.590631
14qI-4_x_st 3.245741
14qI-5_st 2.613702
14qI-6_st 2.724362
14qI-6_x_st 2.475513
14qI-7_st 3.338938
14qI-8_st 0.8097149
14qI-8_x_st 1.281501
14qI-9_x_st 2.373265
14qII-10_st 1.071254
14qII-10_x_st 1.272699
14qII-11_st 1.770588
14qII-11_x_st 1.483106
14qII-12_st 2.73826
14qII-12_x_st 3.43095

Total number of rows: 25015

Table truncated, full table size 634 Kbytes.




Supplementary file Size Download File type/resource
GSM1300460_41_PBx_1171_miRNA.CEL.gz 913.2 Kb (ftp)(http) CEL
Processed data included within Sample table

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