|
| Status |
Public on May 01, 2015 |
| Title |
CCR2-neg-1 |
| Sample type |
RNA |
| |
|
| Source name |
FACS-sorted CCR2- HSC
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
cell type: hematopoietic stem cells (HSC)
cell population: CCR2- HSC
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Arcturus PicoPure RNA extraction kit (Applied Biosystems)
|
| Label |
biotin
|
| Label protocol |
5 ng of total RNA per sample were used as input to the Ovation Pico WTA System V2 (NuGEN)
|
| |
|
| Hybridization protocol |
According to the manufacturer's instructions (Affymetrix)
|
| Scan protocol |
According to the manufacturer's instructions (Affymetrix)
|
| Data processing |
R/Bioconductor, RMA. These six CEL files were normalized as part of a larger batch, so separate normalization may yield slightly different results.
|
| |
|
| Submission date |
Jan 06, 2014 |
| Last update date |
May 01, 2015 |
| Contact name |
Kamila Naxerova |
| Organization name |
Massachusetts General Hospital / Harvard Medical School
|
| Department |
Center For Systems Biology
|
| Street address |
149 13th Street
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE53827 |
CCR2+ vs. CCR2- murine hematopoietic stem cells |
|
