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Sample GSM1303182 Query DataSets for GSM1303182
Status Public on Mar 16, 2014
Title 104 years old Male
Sample type RNA
 
Source name 104 years old Male
Organism Homo sapiens
Characteristics age: 104 years old
Sex: Male
Growth protocol The postmortem brain tissue samples were neuropathologically normal for age, and were derived from non-demented individuals. Tissue was procured in accordance with institutional guidelines. Human cortical grey matter samples were dissected from the frontal pole (Brodmann area 9, 10), and were snap frozen in liquid nitrogen and stored at -85ºC. Human brain samples with a tissue pH > 6.5 were used to exclude prolonged terminal hypoxia
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Dissected cortical grey mater was cut into small pieces in the frozen states and ~70 mg was homogenized immediately in Trizol (Gibco) and RNA was isolated. RNA that was intact by electrophoresis and had an A260/A280 ratio ≥ 1.9 was used for cDNA synthesis. cDNA synthesis, cRNA synthesis and fragmentation, and preparation of the hybridization cocktail were carried out according to the Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual)
 
Hybridization protocol Hybridizations were performed in the Genechip hybridization over 640 (60vrpm) for 16 hrs at 45ºC. The probe arrays were then washed, stained in the GeneChip Fluidics Station 400 operated by GeneChip software following the appropriate fluidics protocols.
Scan protocol Affymetrix Microarray Suite 5.0 was utilized to generate Affymetrix present/absent calls. Probe-level Liner Model (PLM) was used to normalize the arrays at probe level and compute model-based expression values using the PM only model. The dChip software www.dchip.org, [3] was used for clustering visualization.
Description 104 years old Male
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Jan 08, 2014
Last update date Jun 26, 2019
Contact name Tun-Hsiang Yang
E-mail(s) yangth@gmail.com
Organization name HMS
Department Genetics
Lab Yankner
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL570
Series (1)
GSE53890 REST and Stress Resistance in Aging and Alzheimer’s Disease
Relations
Reanalyzed by GSE133349

Data table header descriptions
ID_REF
VALUE log2-MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 8.881487772
1053_at 4.775444254
117_at 5.121470259
121_at 7.273366198
1255_g_at 4.387236159
1294_at 5.761946432
1316_at 5.305798403
1320_at 4.364463725
1405_i_at 3.474004425
1431_at 4.67504194
1438_at 5.179193073
1487_at 5.981990559
1494_f_at 5.237085585
1552256_a_at 6.773061741
1552257_a_at 7.216926521
1552258_at 4.417814648
1552261_at 5.086305335
1552263_at 3.312597378
1552264_a_at 6.30730753
1552266_at 4.092246446

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1303182_104M.CEL.gz 7.7 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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