|
| Status |
Public on Jan 16, 2014 |
| Title |
Control_NPCs_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
NPCs treated retrovirus control
|
| Organism |
Mus musculus |
| Characteristics |
strain: ICR
age: embryonic day 12
tissue: embryonic cortical brain
|
| Treatment protocol |
Retroviral vectors expressing Nurr1 or Foxa2 were constructed by inserting the respective cDNA into pCL. The empty pCL vector was used as a negative control. For viral transduction, NPCs cultured in vitro were incubated with the viral supernatant for 2 hours, followed by a medium change.
|
| Growth protocol |
Neural precursor cells (NPCs) were isolated and cultured from mouse cortices at embryonic days 12. NPCs were proliferated with bFGF (20 ng/m) and EGF (20 ng/ml) in serum-free N2 medium supplemented with ascorbic acid and B27 for expasion. Viral transductions were carried out in the proliferating cultures as described in the treatment protocol. Differentiation of transduced NPCs was induced by withdrawing bFGF and EGF for 2 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol reagent according to the instructions of manufacturer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R. Array probes that have detection p-value > 0.05 (similar to signal to noise) in over 50% samples were filtered out before normalization. (We applied a filtering criterion for data analysis; higher signal value was required to obtain a detection p-value < 0.05) Selected gene signal value was transformed by logarithm and normalized by quantile method.
|
| |
|
| Submission date |
Jan 15, 2014 |
| Last update date |
Jan 16, 2014 |
| Contact name |
Sang-Hun Lee |
| E-mail(s) |
leesh@hanyang.ac.kr
|
| Phone |
82-2-2220-0625
|
| Organization name |
College of medicine, Hanyang University
|
| Department |
biochemistry and molecular biology
|
| Street address |
222 Wangsimni-ro, Seongdong-gu
|
| City |
Seoul |
| ZIP/Postal code |
133-791 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE54086 |
Microarray analysis of Nurr1 and Foxa2 synergisitic effect for DA neuron induction |
|
